Fujita A, Kobayashi M, Ueda H, Fujita N, Ueda K
Department of Pediatrics, Hiroshima University School of Medicine, Japan.
Pediatr Res. 1996 Sep;40(3):388-92. doi: 10.1203/00006450-199609000-00004.
We compared the in vitro response of myeloid progenitor cells [colony-forming units of culture (CFU-C)] that were prepared from human umbilical cord blood to the administration of the combination of granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) versus that of CFU-C obtained from normal human bone marrow. Progenitors were purified according to CD34 expression; the number and size of colonies were evaluated by culture in agar or methylcellulose, respectively. In the presence of G-CSF alone, the mean number of colonies was significantly greater in the bone marrow culture versus that of cord blood. SCF alone bad little effect on colony formation, but in the presence of optimal or suboptimal concentrations of G-CSF, SCF significantly increased colony formation from both cell sources. Its effect on cord blood significantly exceeded that on bone marrow. SCF used in combination with G-CSF significantly increased the size of the colonies with cord blood CFU-C; this effect was less marked with bone marrow CFU-C. The percentage of cells that expressed c-Kit, the SCF receptor, did not appear to differ between the two sources of CFU-C. Results indicate that cord blood CFU-C showed a greater response to SCF in combination with G-CSF than did bone marrow CFU-C.
我们比较了从人脐带血制备的髓系祖细胞[培养集落形成单位（CFU-C）]与从正常人骨髓获得的CFU-C，对粒细胞集落刺激因子（G-CSF）和干细胞因子（SCF）联合给药的体外反应。根据CD34表达纯化祖细胞；分别通过在琼脂或甲基纤维素中培养来评估集落的数量和大小。单独使用G-CSF时，骨髓培养中的集落平均数明显高于脐带血培养中的集落平均数。单独使用SCF对集落形成几乎没有影响，但在存在最佳或次最佳浓度的G-CSF时，SCF可显著增加两种细胞来源的集落形成。其对脐带血的作用明显超过对骨髓的作用。SCF与G-CSF联合使用可显著增加脐带血CFU-C集落的大小；对骨髓CFU-C的这种作用不太明显。表达SCF受体c-Kit的细胞百分比在两种CFU-C来源之间似乎没有差异。结果表明，与骨髓CFU-C相比，脐带血CFU-C对SCF与G-CSF联合使用的反应更大。
