[Intrahepatic antioxidative enzymes during hemorrhagic shock].

A histochemical study on the distribution of intrahepatic Cu-Zn SOD, Mn SOD and catalase was made using rat models for hemorrhagic shock. Hemorrhagic shock with an average blood pressure of 40 mmHg was created and blood was circulated again two hours later. Before the shock, Cu-Zn SOD was homogeneously distributed in hepatic lobuli; and, in cells, it was predominantly distributed in cytoplasms. With the progress of the shock, the number of Cu-Zn SOD began to decrease in the region around the central veins of liver; and, in cells, it came to be distributed predominantly in cell nuclei. Even after the circulation of blood, similar changes were observed. The changes of the distribution of Mn SOD were similar to those of Cu-Zn SOD. As to catalase before the shock, it was homogeneously distributed in lobuli; and, in cells, it was distributed in cytoplasms in the form of a minute granule. Unlike the cases of SODs, no significant change of the distribution of catalase was observed even after the progress of the shock. These results suggest that the functional decline in the protective system against active oxygen also relates to the cause of the progress of the cell injury even after the circulation of blood.