在乳酸乳球菌乳酸亚种双乙酰乳酸亚种的质粒pCIT264中鉴定出的一种类插入序列元件的特性分析。

Characterization of an insertion sequence-like element identified in plasmid pCIT264 from Lactococcus lactis subsp. lactis biovar diacetylactis.

作者信息

Magni C, de Felipe F L, López P, de Mendoza D

机构信息

Programa Multidisciplinario de Biología Experimental (PROMUBIE-CONICET), Universidad Nacional de Rosario, Argentina.

出版信息

FEMS Microbiol Lett. 1996 Mar 1;136(3):289-95. doi: 10.1111/j.1574-6968.1996.tb08063.x.

DOI:10.1111/j.1574-6968.1996.tb08063.x

PMID:8867382

Abstract

Plasmid pCIT264 from Lactococcus lactis subsp. lactis biovar diacetylactis (L. diacetylactis) contains an insertion sequence (IS)-like element located in the citrate utilization (citQRP) cluster. This 967-nucleotide long element is bounded by 17 bp perfect inverted repeats and contains an open reading frame (ORF1) composed of 296 codons, which could encode a transposase. Expression of the IS from pCIT264 generates two mRNAs of 2900 and 1900 nucleotides. The transcription is driven by the P3 promoter, composed of a -10 region located at the right end of the IS and of a -35 region positioned downstream of this element. The IS-like element (IS982) is present in seven copies in the L.diacetylactis genome. The copy present in pCIT264 is highly stable and does not promote rearrangements of the cit cluster. We suggest that the stable maintenance of the IS-like element in pCIT264 could be due to a translational control of the putative transposase by an antisense RNA.

摘要

来自乳酸乳球菌乳酸亚种双乙酰乳酸变种（L. diacetylactis）的质粒pCIT264含有一个位于柠檬酸盐利用（citQRP）簇中的类插入序列（IS）元件。这个967个核苷酸长的元件由17个碱基对的完美反向重复序列界定，包含一个由296个密码子组成的开放阅读框（ORF1），它可能编码一种转座酶。来自pCIT264的IS的表达产生了两种2900和1900个核苷酸的mRNA。转录由P3启动子驱动，该启动子由位于IS右端的 -10区域和位于该元件下游的 -35区域组成。类IS元件（IS982）在L. diacetylactis基因组中以七个拷贝存在。存在于pCIT264中的拷贝高度稳定，不会促进cit簇的重排。我们认为pCIT264中类IS元件的稳定维持可能是由于反义RNA对推定转座酶的翻译控制。