Schneiderka P, Bezdícková D, Uhrová J
Department of Clinical Biochemistry, Charles University, Praha, Czech Republic.
Sb Lek. 1994;95(4):363-72.
Some analytical properties of chemiluminescent immunoassays (ChLIA) for the estimation of total triiodothyronine (T3), total thyroxine (T4) and thyrotropine (TSH) in serum were studied and compared with radioimmunoanalysis (RIA) as a reference method. Measurement range of ChLIA for T3 is lower, for T4 is equivalent and for TSH is greater than by RIA methods. Analytical sensitivity of ChLIA is better for all three analytes. Also precision of ChLIA is much better. ChLIA method seems to be more resistant to lipaemia. When compared to secondary reference materials, i.e. to commercial control sera, higher accuracy can be evaluated. However, adjustment of the control sera and analytical methods to different primary standards and calibrators, resulting in disagreement of results with asigned values, seems to be evident. With respect to the accuracy of ChLIA further study should be performed involving primary standards. Other interferences except of lipaemia, which we refer to in our work, and problems of specificity also need to be elucidated.
研究了用于测定血清中总三碘甲状腺原氨酸（T3）、总甲状腺素（T4）和促甲状腺激素（TSH）的化学发光免疫分析（ChLIA）的一些分析特性，并与作为参考方法的放射免疫分析（RIA）进行了比较。ChLIA对T3的测量范围低于RIA方法，对T4的测量范围与RIA方法相当，对TSH的测量范围则大于RIA方法。ChLIA对所有三种分析物的分析灵敏度更高。此外，ChLIA的精密度也更好。ChLIA方法似乎对脂血更具抗性。与二级参考物质（即商业对照血清）相比，可以评估出更高的准确性。然而，对照血清和分析方法对不同一级标准品和校准品的调整导致结果与赋值不一致，这一点似乎很明显。关于ChLIA的准确性，应进一步开展涉及一级标准品的研究。除了我们在工作中提到的脂血外，其他干扰因素以及特异性问题也需要阐明。
