Montesino R, Cremata J, Rodríguez M, Besada V, Falcón V, de la Fuente J
Division of Industrial Biotechnology, Centro de Ingeniería Genética y Biotechnología, La Habana, Cuba.
Biotechnol Appl Biochem. 1996 Feb;23(1):23-8.
In the present paper we report the biochemical characteristics of the recombinant tick (Boophilus microplus) gut antigen Bm86 that previously has been cloned, expressed and recovered at high levels in the methylotrophic yeast Pichia pastoris. The results demonstrate that rBm86 had a modification at position 92 (Thr replaced by Ile) and aggregated, forming particles ranging between 17 and 40 nm. The rBm86 was N-glycosylated, having at least two non-glycosylated sequons (Asn-329 and Asn-363) and a ratio of only 0.4/65 (free Cys/total Cys)/mol of protein.
在本论文中，我们报告了重组蜱（微小牛蜱）肠道抗原Bm86的生化特性，该抗原先前已在甲基营养型酵母毕赤酵母中克隆、表达并大量回收。结果表明，rBm86在第92位发生修饰（苏氨酸被异亮氨酸取代）并聚集，形成直径在17至40纳米之间的颗粒。rBm86进行了N-糖基化，至少有两个非糖基化序列（天冬酰胺-329和天冬酰胺-363），每摩尔蛋白质的游离半胱氨酸/总半胱氨酸比例仅为0.4/65。
