Naganuma H, Sasaki A, Satoh E, Nagasaka M, Nakano S, Isoe S, Nukui H
Department of Neurosurgery, Yamanashi Medical University.
Neurol Med Chir (Tokyo). 1996 Mar;36(3):145-50. doi: 10.2176/nmc.36.145.
Malignant glioma cells secrete transforming growth factor-beta (TGF-beta) which has potent immunosuppressive properties. We investigated the effect of interleukin-1 beta (IL-1 beta) on TGF-beta secretion from malignant glioma cells in vitro. T98G glioblastoma cells were treated with various doses of IL-1 beta and the TGF-beta activity in the supernatant was determined using a specific bioassay. Six other human malignant glioma cell lines were also treated with 1000 U/ml of IL-1 beta, and the TGF-beta activity in the supernatants was determined. The effect of IL-1 beta on the growth of tumor cells was also assessed by a bioassay using crystal violet which reflects the actual cell number in the plate wells. IL-1 beta treatment resulted in inhibition of TGF-beta secretion in two malignant glioma cell lines. TGF-beta secretion from T98G cells was suppressed by IL-1 beta in a dose-related manner. However, IL-1 beta treatment resulted in an obvious increase (> 20%) of TGF-beta secretion in two tumor lines, and a slight increase (< 20%) in three tumor lines. IL-1 beta did not affect the growth of four malignant glioma cell lines, and only slightly affected the growth of the other three cell lines. IL-1 beta modulates TGF-beta secretion from malignant glioma cells, but not in a consistent way.
恶性胶质瘤细胞分泌具有强大免疫抑制特性的转化生长因子-β（TGF-β）。我们在体外研究了白细胞介素-1β（IL-1β）对恶性胶质瘤细胞分泌TGF-β的影响。用不同剂量的IL-1β处理T98G胶质母细胞瘤细胞，并使用特定的生物测定法测定上清液中的TGF-β活性。还用1000 U/ml的IL-1β处理另外六种人类恶性胶质瘤细胞系，并测定上清液中的TGF-β活性。还通过使用结晶紫的生物测定法评估IL-1β对肿瘤细胞生长的影响，该方法反映平板孔中的实际细胞数量。IL-1β处理导致两种恶性胶质瘤细胞系中TGF-β分泌受到抑制。T98G细胞的TGF-β分泌被IL-1β以剂量相关的方式抑制。然而，IL-1β处理导致两种肿瘤细胞系中TGF-β分泌明显增加（>20%），三种肿瘤细胞系中略有增加（<20%）。IL-1β不影响四种恶性胶质瘤细胞系的生长，仅对其他三种细胞系的生长有轻微影响。IL-1β可调节恶性胶质瘤细胞的TGF-β分泌，但方式并不一致。
