Muguerza B, Lecároz C, Picardi A, Castilla-Cortázar I, Quiroga J, Cemborain A, Prieto J, Santidrián S
Departamento de Fisiología Humana, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.
Rev Esp Fisiol. 1996 Jun;52(2):113-9.
In order to search for an experimental model to further investigate the osteopenia associated to liver cirrhosis (LC), this study has been focused on investigating the occurrence of bone disorders in male rats to which LC histologically confirmed was induced through the validated procedure of CCl4 inhalation. Length, anteroposterior and lateromedial diameters, densitometry, mechanical stress resistance, hydroxyproline (OHprol) and calcium and phosphate contents were measured in femurs from control (n = 10) and liver cirrhosis rats (n = 10). It has been found that femurs from liver cirrhosis rats showed a significant reduction (p < 0.01) in bone weight (0.254 +/- 0.003 vs 0.230 +/- 0.004 g/100 g b.w.), anteroposterior (4.08 +/- 0.06 vs 3.69 +/- 0.05 mm) and lateromedial (5.33 +/- 0.05 vs 5.08 +/- 0.04 mm, p < 0.05) diameters, resistance to mechanical stress (405.8 +/- 9.5 vs 332.5 +/- 9.1 N) and total densitometry (0.416 +/- 0.005 vs 0.381 +/- 0.004 g/cm2). However, no significant differences were observed in bone length, calcium, OHprol and phosphate (all expressed as mg/100 mg fresh bone tissue) contents. Therefore, the proteins matrix to mineral contents ratio was not altered. These results indicate that in this model of experimental liver cirrhosis there is osteopenia characterized by bone frailty and reduced thickness, and it could offer an experimental model to study bone changes associated to liver cirrhosis.
为了寻找一种实验模型以进一步研究与肝硬化（LC）相关的骨质减少，本研究着重调查经四氯化碳吸入的经验证程序诱导出组织学确诊的LC的雄性大鼠中骨疾病的发生情况。对对照组（n = 10）和肝硬化大鼠（n = 10）的股骨测量长度、前后径和内外径、骨密度、抗机械应力能力、羟脯氨酸（OHprol）以及钙和磷含量。结果发现，肝硬化大鼠的股骨在骨重量（0.254±0.003 vs 0.230±0.004 g/100 g体重）、前后径（4.08±0.06 vs 3.69±0.05 mm）和内外径（5.33±0.05 vs 5.08±0.04 mm，p < 0.05）、抗机械应力能力（405.8±9.5 vs 332.5±9.1 N）和总骨密度（0.416±0.005 vs 0.381±0.004 g/cm²）方面均出现显著降低（p < 0.01）。然而，在骨长度、钙、OHprol和磷（均以mg/100 mg新鲜骨组织表示）含量方面未观察到显著差异。因此，蛋白质基质与矿物质含量的比率未改变。这些结果表明，在这种实验性肝硬化模型中存在以骨脆弱和厚度减小为特征的骨质减少，并且它可为研究与肝硬化相关的骨变化提供一种实验模型。
