de Romeuf C, Mazurier C
Laboratoire de Recherche sur l'Hémostase, Laboratoire Français du Fractionnement et des Biotechnologies, Lille, France.
Thromb Res. 1996 Aug 15;83(4):287-98. doi: 10.1016/0049-3848(96)00137-5.
Quantitative or qualitative assay of platelet von Willebrand factor (vWF) is required for the classification of patients suffering from von Willebrand disease (vWD). The major interest of this classification is the administration of specific and appropriate treatment to each category of vWD patients. However, because platelet lysate is prepared from washed platelets, the analysis of platelet vWF is not performed in routine. To overcome this problem, platelet lysates were prepared either with a new method which does not require washing procedure or with the established protocol, then respective platelet vWF characteristics were compared. Our data show that the two methods provide comparable information required for the characterization of types 1 and 2 vWD patients. With this new procedure, complete phenotypic data including the characteristics of platelet vWF can be obtained from most of the vWD patients.
对患有血管性血友病（vWD）的患者进行分类需要对血小板血管性血友病因子（vWF）进行定量或定性检测。这种分类的主要意义在于为每类vWD患者提供特定且合适的治疗。然而，由于血小板裂解物是由洗涤后的血小板制备而成，血小板vWF的分析并非常规操作。为克服这一问题，采用无需洗涤步骤的新方法或既定方案制备血小板裂解物，然后比较各自血小板vWF的特征。我们的数据表明，这两种方法为1型和2型vWD患者的特征描述提供了可比的信息。通过这种新方法，可从大多数vWD患者获得包括血小板vWF特征在内的完整表型数据。
