Sassoè-Pognetto M, Feigenspan A, Bormann J, Wässle H
Max-Planck-Institut für Hirnforschung, Neuroanatomische Abteilung, Frankfurt, Germany.
Vis Neurosci. 1996 Jul-Aug;13(4):759-71. doi: 10.1017/s0952523800008634.
Vertical slices of postnatal day 6 (P6) rat retina were cut and cultured using the roller-tube technique. The organotypic differentiation during a culture period of up to 30 days has been described in a previous study (Feigenspan et al., 1993a). Here we concentrated on the synaptic organization in the retinal slice culture. Electron microscopy revealed the presence of ribbon synapses in the outer plexiform layer and conventional and ribbon synapses in the inner plexiform layer. Immunofluorescence with antibodies that recognize specific subunits of GABAA or glycine receptors revealed a punctate distribution of the receptors. They were aggregated in "hot spots" that correspond to a concentration of receptors at postsynaptic sites. Different isoforms of GABAA and glycine receptors occurred in the slice cultures. The experiments show that there is a differentiation of synapses and a diversity of transmitter receptors in the slice cultures that is comparable to the in vivo retina.
切取出生后第6天（P6）大鼠视网膜的垂直切片，采用滚管技术进行培养。先前的一项研究（Feigenspan等人，1993a）已描述了长达30天培养期内的器官型分化。在此，我们着重研究视网膜切片培养中的突触组织。电子显微镜显示在外丛状层存在带状突触，在内丛状层存在常规突触和带状突触。用识别GABAA或甘氨酸受体特定亚基的抗体进行免疫荧光检测，显示受体呈点状分布。它们聚集在“热点”区域，这些区域对应于突触后位点处受体的集中分布。切片培养中出现了不同亚型的GABAA和甘氨酸受体。实验表明，切片培养中的突触分化和递质受体多样性与体内视网膜相当。
