Kelkar S D, Ray P G, Bedekar S S
National Institute of Virology, Pune, India.
J Diarrhoeal Dis Res. 1996 Jun;14(2):101-6.
In the western literature, four G serotypes (G1-G4) of human rotaviruses have been found to be of a major epidemiological importance. During the analysis of rotavirus serotypes from faecal samples in Pune, over 50% of specimens could not be serotyped with the available monoclonal antibodies against G1-G4 serotypes. The results prompted to look for the prevalence of neutralizing antibodies against serotypes other than the major human serotypes (G1-G4) in adults. Neutralizing antibodies against animal rotavirus serotype, viz. G3, G6 and G10, and human rotavirus serotype G8 were determined in adult sera, by a modified technique, which is ELISA-based and mechanized. The results showed that, of the 68 sera tested at 1:100 dilution, 65 (95.58%) were reactive for G3 (SA-11), 52 (76.47%) for G6 (Bovine Lincoln), 6 (8.82%) for G10 (B223), and 40 (58.82%) for G8 (M69) serotypes. It appears that the prevalence of rotaviruses in India may be quite different from that in the developed countries.
在西方文献中，已发现四种人类轮状病毒G血清型（G1 - G4）具有重要的流行病学意义。在对浦那粪便样本中的轮状病毒血清型进行分析时，超过50%的样本无法用现有的针对G1 - G4血清型的单克隆抗体进行血清分型。这一结果促使人们去研究成年人中针对主要人类血清型（G1 - G4）以外血清型的中和抗体的流行情况。采用一种基于酶联免疫吸附测定（ELISA）的改良机械化技术，对成人血清中针对动物轮状病毒血清型G3、G6和G10以及人类轮状病毒血清型G8的中和抗体进行了检测。结果显示，在68份以1:100稀释度检测的血清中，65份（95.58%）对G3（SA - 11）呈反应性，52份（76.47%）对G6（牛林肯株）呈反应性，6份（8.82%）对G10（B223）呈反应性，40份（58.82%）对G8（M69）血清型呈反应性。看来印度轮状病毒的流行情况可能与发达国家有很大不同。
