Obrenovitch T P, Zilkha E
Department of Neurological Surgery, Institute of Neurology, London, U.K.
Acta Neurochir Suppl. 1996;67:21-3. doi: 10.1007/978-3-7091-6894-3_4.
It is accepted that the ionic composition of the medium perfused through a microdialysis probe should match that of the extracellular fluid (ECF) under physiological conditions. In contrast, the possibility that control artificial cerebrospinal fluid may influence the experimental or pathological conditions under study, by buffering changes in the ECF composition, has been neglected. Spreading depression (SD) is a propagating transient suppression of electrical activity due to cellular depolarization which may contribute to neuronal damage in focal ischaemia, and underlie the migraine aura Here we report that microdialysis markedly inhibits SD propagation, by buffering the sudden increase in extracellular K+ associated with this event. This effect is independent of the microdialysis flow rate and does not result from tissue injury following probe implantation. This finding clearly illustrates that microdialysis can influence the pathological conditions under investigation.
人们普遍认为，在生理条件下，通过微透析探针灌注的介质的离子组成应与细胞外液（ECF）的离子组成相匹配。相比之下，对照人工脑脊液可能通过缓冲ECF组成的变化来影响所研究的实验或病理状况，这种可能性一直被忽视。扩散性抑制（SD）是由于细胞去极化导致的电活动的传播性短暂抑制，它可能导致局灶性缺血中的神经元损伤，并构成偏头痛先兆的基础。在此我们报告，微透析通过缓冲与此事件相关的细胞外K+的突然增加，显著抑制SD的传播。这种效应与微透析流速无关，也不是探针植入后组织损伤的结果。这一发现清楚地表明，微透析可以影响所研究的病理状况。
