Lolova I S, Davidoff M S, Yakimoff N A
Institute of Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Acta Physiol Pharmacol Bulg. 1996;22(1):7-16.
Immunohistochemistry and morphometry were used to study the age-related changes in the vasopressin (AVP) and oxytocin (OXT) nerve cells in the paraventricular (PVN), supraoptic (SON) and suprachiasmatic (SCN) nuclei of 3-, 11- and 28-month-old rats. The results showed a statistically significant reduction in the mean number of AVP cells in the PVN, SON and SCN, and of OXT cells in the PVN with advancing age. Different age-related changes in the mean size of the immunoreactive cells were found in the three nuclei: a significant and transitory increase in the AVP and OXT cell sizes in the PVN, a tendency towards increasing the AVP and OXT cell sizes in the SON, and a significant and gradual decrease in the AVP cell size in the SCN. The combination of the morphometric data and staining patterns of the AVP and OXT perikarya and fibers in the PVN and SON pointed to an increased transport of AVP and OXT in 11-month-old rats as well as to a decreased production of these peptides in the PVN of 28-month-old rats. Taken together the staining pattern and the morphometric results showed a progressive loss of AVP cells in the SCN in aging.
采用免疫组织化学和形态计量学方法，研究3月龄、11月龄和28月龄大鼠室旁核（PVN）、视上核（SON）和视交叉上核（SCN）中加压素（AVP）和催产素（OXT）神经细胞的年龄相关变化。结果显示，随着年龄增长，PVN、SON和SCN中AVP细胞的平均数量以及PVN中OXT细胞的平均数量均有统计学意义的减少。在这三个核中发现了免疫反应性细胞平均大小的不同年龄相关变化：PVN中AVP和OXT细胞大小显著且短暂增加，SON中AVP和OXT细胞大小有增加趋势，SCN中AVP细胞大小显著且逐渐减小。PVN和SON中AVP和OXT核周体及纤维的形态计量数据和染色模式表明，11月龄大鼠中AVP和OXT的转运增加，28月龄大鼠PVN中这些肽的产生减少。综合染色模式和形态计量结果表明，衰老过程中SCN中AVP细胞逐渐丢失。
