染色质羟基磷灰石热层析过程中小鼠卫星DNA和核糖体DNA的变性

Denaturation of mouse satellite and ribosomal DNA during hydroxyapatite thermal chromatography of chromatin.

作者信息

Pashev I G

出版信息

Mol Biol Rep. 1977 Jun;3(4):297-303. doi: 10.1007/BF00368300.

Abstract

Mouse DNA and chromatin were melted on hydroxyapatite and the denaturation profiles of ribosomal and satellite DNAs were followed by hybridization with their complementary RNAs. Neither ribosomal nor bulk DNA had significantly different melting profiles in chromatin as compared to DNA. However, most of satellite DNA eluted at higher temperature from chromatin than from purified DNA. One explaination for the higher melting temperature of mouse satellite DNA in chromatin suggests that the complex between this particular DNA component and at least some proteins in chromatin is more stable than the average DNA-protein interaction.

摘要

将小鼠的DNA和染色质在羟基磷灰石上进行熔解，通过与核糖体DNA和卫星DNA的互补RNA杂交来追踪它们的变性图谱。与DNA相比，染色质中的核糖体DNA和总DNA的熔解图谱均无显著差异。然而，大多数卫星DNA从染色质中洗脱的温度高于从纯化DNA中洗脱的温度。对染色质中小鼠卫星DNA熔解温度较高的一种解释是，这种特定的DNA组分与染色质中至少一些蛋白质之间的复合物比平均的DNA-蛋白质相互作用更稳定。