大肠杆菌、牛、小鼠、大鼠和鸡的DNA的微分DNA解链曲线与CsCl密度曲线的比较。

A comparison of the differential DNA melting profiles with the CsCl density profiles of DNA from Escherichia coli, cow, mouse, rat and chicken.

作者信息

Mayfield J E

出版信息

Biochim Biophys Acta. 1977 Jul 15;477(2):97-101. doi: 10.1016/0005-2787(77)90225-8.

Abstract

Moderate resolution thermal denaturation profiles are presented for the purified DNAs from Escherichia coli, cow, mouse, rat and chicken. All show multiple thermal transitions indicative of large blocks of DNA with very similar base composition. The eucaryotes all have much more of this kind of DNA than does E. coli. The satellite DNAs of cow and mouse are clearly visible and it is likely that the other transitions represent additional families of repeated DNA.

摘要

本文展示了来自大肠杆菌、牛、小鼠、大鼠和鸡的纯化DNA的中等分辨率热变性图谱。所有图谱均显示出多个热转变，表明存在碱基组成非常相似的大片段DNA。真核生物中这种DNA的含量比大肠杆菌多得多。牛和小鼠的卫星DNA清晰可见，其他转变可能代表了重复DNA的其他家族。