类风湿性关节炎患者中性粒细胞中溶酶体酶的释放能力。

The releasability of lysosomal enzymes from neutrophil leukocytes in patients with rheumatoid arthritis.

作者信息

Marinò I, Columbo M, Marone G

机构信息

Department of Medicine, University of Naples Federico II, School of Medicine, Italy.

出版信息

Clin Exp Rheumatol. 1996 Jul-Aug;14(4):387-94.

PMID:8871837

Abstract

OBJECTIVE

To study the enzyme content and the "releasability" of lysosomal enzymes (lysozyme and beta-glucuronidase) in neutrophils purified from peripheral blood of patients with rheumatoid arthritis (RA) or normal subjects.

METHODS

Neutrophils were obtained from 13 patients (10 women and 3 men) with rheumatoid arthritis and from 11 healthy subjects (8 women and 3 men). We measured: (1) lysosomal enzyme (lysozyme and beta-glucuronidase) content; (2) spontaneous enzyme release; (3) lysosomal enzyme release after cell challenge with different segretagogues (FMLP, C5a, aggregated IgG, zymosan and Ca2+ ionophore A23187).

RESULTS

The lysosomal enzyme content was not statistically different in control subjects and in patients with RA (7.4 +/- 1.9 vs 6.3 +/- 0.8 micrograms/10(6) neutrophils for lysozyme; 102.9 +/- 16.4 vs 78.9 +/- 11.2 micrograms/10(6) neutrophils for beta-glucuronidase in control and RA subjects, respectively, p = NS). Unstimulated release of lysozyme was significantly lower in RA patients (3.8 +/- 1.1%) when compared to control subjects (9.5 +/- 2.1%) (p < 0.05). In contrast, spontaneous release of beta-glucuronidase did not differ in the two groups (5.5 +/- 0.9% and 3.8 +/- 1.1% in control and RA subjects, respectively). Enzyme release induced by FMLP (3 x 10(-9)-3 x 10(-7) M), C5a (10(-8)-10(-7) M), aggregated IgG (0.1-0.6 mg/ml), or Ca2+ ionophore A23187 (0.1-1 microgram/ml) did not differ statistically in the two groups of subjects. Neutrophil stimulation by serum-treated zymosan, at the concentration of 0.3 mg/ml, induced a release of lysozyme that was significantly higher in patients with RA when compared to control subjects (p < 0.05), whereas zymosan-activated beta-glucuronidase secretion was similar in the two donor populations.

CONCLUSIONS

This study suggests that the contribution of leukocytes to the inflammatory processes typical of RA does not depend on an altered "releasability" of preformed mediators from peripheral blood neutrophils.

摘要

目的

研究从类风湿关节炎（RA）患者或正常受试者外周血中纯化的中性粒细胞中溶酶体酶（溶菌酶和β-葡萄糖醛酸酶）的含量及“释放能力”。

方法

从13例类风湿关节炎患者（10名女性和3名男性）和11名健康受试者（8名女性和3名男性）中获取中性粒细胞。我们测量了：（1）溶酶体酶（溶菌酶和β-葡萄糖醛酸酶）含量；（2）酶的自发释放；（3）用不同促分泌剂（FMLP、C5a、聚集的IgG、酵母聚糖和Ca2+离子载体A23187）刺激细胞后溶酶体酶的释放。

结果

对照组和RA患者的溶酶体酶含量无统计学差异（溶菌酶：对照组为7.4±1.9μg/10⁶中性粒细胞，RA患者为6.3±0.8μg/10⁶中性粒细胞；β-葡萄糖醛酸酶：对照组为102.9±16.4μg/10⁶中性粒细胞，RA患者为78.9±11.2μg/10⁶中性粒细胞，p=无显著性差异）。与对照组（9.5±2.1%）相比，RA患者溶菌酶的未刺激释放显著降低（3.8±1.1%）（p<0.05）。相比之下，两组中β-葡萄糖醛酸酶的自发释放无差异（对照组和RA患者分别为5.5±0.9%和3.8±1.1%）。FMLP（3×10⁻⁹ - 3×10⁻⁷M）、C5a（10⁻⁸ - 10⁻⁷M）、聚集的IgG（0.1 - 0.6mg/ml）或Ca2+离子载体A23187（0.1 - 1μg/ml）诱导的酶释放在两组受试者中无统计学差异。浓度为0.3mg/ml的经血清处理的酵母聚糖刺激中性粒细胞后，RA患者溶菌酶的释放显著高于对照组（p<0.05），而酵母聚糖激活的β-葡萄糖醛酸酶分泌在两组供体群体中相似。