Dumas S, Pequignot J M, Ghilini G, Mallet J, Denavit-Saubié M
Laboratoire de Génétique Moléculaire de la Neurotransmission et Processus Neurodégénératifs, UMR 9923 CNRS, Hôpital de La Pitié-Salpêtrière, Paris, France.
Brain Res Mol Brain Res. 1996 Sep 1;40(2):188-94. doi: 10.1016/0169-328x(96)00050-2.
The aim of this study was to define the influence of long-term hypoxia on gene expression of tyrosine hydroxylase (TH) in the rat nucleus tractus solitarius (NTS). Animals were exposed to normobaric hypoxia (10% O2 in nitrogen) for 2 weeks. At this time, the hypoxia-induced hyperventilation reached a plateau, indicating ventilatory acclimatization. In horizontal brainstem sections, hypoxia-induced changes in TH protein and TH mRNA were assessed by immunocytochemistry and in-situ hybridization, respectively. Long-term hypoxia increased TH mRNA levels seen as both an increase in the number of grains per cell and an extension of the labeled area. The highest degree of labeling was found selectively located in caudal NTS. Hypoxia also enhanced TH immunoreactivity in the caudal NTS but this labeling extended more rostrally than that of TH mRNA. The data suggest that there is an hypoxia-induced plasticity of gene expression at the gene level in the NTS, which is associated with ventilatory acclimatization. The hypoxia model described in this study may serve as a framework for future regulatory studies.
本研究的目的是确定长期低氧对大鼠孤束核（NTS）中酪氨酸羟化酶（TH）基因表达的影响。将动物置于常压低氧环境（氮气中含10%氧气）2周。此时，低氧诱导的过度通气达到平台期，表明通气适应。在水平脑干切片中，分别通过免疫细胞化学和原位杂交评估低氧诱导的TH蛋白和TH mRNA的变化。长期低氧增加了TH mRNA水平，表现为每个细胞中颗粒数量的增加以及标记区域的扩大。最高程度的标记选择性地位于尾侧NTS。低氧还增强了尾侧NTS中的TH免疫反应性，但这种标记比TH mRNA的标记向更靠前的方向延伸。数据表明，NTS在基因水平存在低氧诱导的基因表达可塑性，这与通气适应有关。本研究中描述的低氧模型可作为未来调控研究的框架。
