Hernández-López S, Flores G, Rosales M G, Sierra A, Martínez-Fong D, Aceves J
Departamento de Fisiología, Biofísica y Neurociencias, CINVESTAV-IPN, México D.F., Mexico.
Neurosci Lett. 1996 Aug 9;213(3):157-60.
The ability of anticholinergic agents microinjected into the subthalamic nucleus to reduce reserpine-induced muscular rigidity was assessed in rats. The electromyographical activity of the gastrocnemius-soleus muscle was used as a parameter of muscular rigidity. Reserpine (5 mg/kg i.p.) produced the appearance of electromyographical activity. The muscarinic antagonists M3 (1.27 nmol of 4-DAMP) and M1 (2.36 nmol of pirenzepine) markedly reduced the reserpine-induced electromyographical activity, whereas the M2 antagonist AFDX-116 (2.37 nmol) had no effect. These results suggest that a high cholinergic tone in the subthalamic nucleus is associated with the reserpine-induced muscular rigidity. Moreover, the M3 muscarinic antagonist is more effective than the M1 muscarinic antagonist in reducing the muscular rigidity in reserpinized rats, a model of Parkinson's disease, by blocking the high cholinergic tone in the subthalamic nucleus.
在大鼠中评估了微量注射到丘脑底核的抗胆碱能药物减轻利血平诱导的肌肉强直的能力。将腓肠肌-比目鱼肌的肌电图活动用作肌肉强直的参数。利血平（5毫克/千克腹腔注射）使肌电图活动出现。毒蕈碱拮抗剂M3（1.27纳摩尔的4-DAMP）和M1（2.36纳摩尔的哌仑西平）显著降低了利血平诱导的肌电图活动，而M2拮抗剂AFDX-116（2.37纳摩尔）则没有效果。这些结果表明，丘脑底核中高胆碱能张力与利血平诱导的肌肉强直有关。此外，在帕金森病模型——利血平化大鼠中，通过阻断丘脑底核中的高胆碱能张力，M3毒蕈碱拮抗剂在减轻肌肉强直方面比M1毒蕈碱拮抗剂更有效。
