Ferreira C
Gulbenkian Institute of Science, Oeiras, Portugal.
Virus Res. 1996 Sep;44(1):11-21. doi: 10.1016/0168-1702(96)01334-2.
Northern blot hybridisation was used to study the accumulation of specific cellular mRNAs (ubiquitin and actin) in Vero cells infected with African swine fever virus (ASFV). ASFV modulates the cytoplasmic levels of ubiquitin and actin mRNAs throughout infection. Before viral DNA replication, degradation of ubiquitin mRNAs is dependent on de novo protein synthesis, since treatment with cycloheximide (CH) allowed the accumulation of ubiquitin mRNAs, while treatment with cytosine arabinoside (araC) induced a reduction in ubiquitin transcripts. Nevertheless, viral DNA replication is essential to the final increase observed in ubiquitin mRNA degradation. Furthermore, ubiquitin transcription seems to be tightly related to viral gene transcription, since before viral DNA replication ubiquitin and viral transcripts accumulate at opposite rates. Concerning actin transcription, the first step in actin mRNA degradation does not depend on de novo protein synthesis, since treatment with CH induced a reduction in actin mRNA. The second step in actin mRNA degradation, similarly to ubiquitin, depends on viral DNA replication. Finally, in the present study it has also been shown that ASFV codifies for actin-like genes. This is the first report of a virus encoding an actin-like gene.
采用Northern印迹杂交法研究感染非洲猪瘟病毒（ASFV）的Vero细胞中特定细胞mRNA（泛素和肌动蛋白）的积累情况。在整个感染过程中，ASFV调节泛素和肌动蛋白mRNA的细胞质水平。在病毒DNA复制之前，泛素mRNA的降解依赖于从头合成蛋白质，因为用环己酰亚胺（CH）处理可使泛素mRNA积累，而用阿糖胞苷（araC）处理则导致泛素转录本减少。然而，病毒DNA复制对于泛素mRNA降解中观察到的最终增加至关重要。此外，泛素转录似乎与病毒基因转录密切相关，因为在病毒DNA复制之前，泛素和病毒转录本以相反的速率积累。关于肌动蛋白转录，肌动蛋白mRNA降解的第一步不依赖于从头合成蛋白质，因为用CH处理会导致肌动蛋白mRNA减少。与泛素类似，肌动蛋白mRNA降解的第二步依赖于病毒DNA复制。最后，在本研究中还表明，ASFV编码肌动蛋白样基因。这是关于病毒编码肌动蛋白样基因的首次报道。
