Brand-Saberi B, Müller T S, Wilting J, Christ B, Birchmeier C
Anatomisches Institut, Lehrstuhl II, Albert-Ludwigs-Universität Freiburg, Germany.
Dev Biol. 1996 Oct 10;179(1):303-8. doi: 10.1006/dbio.1996.0260.
The initiating event in the migration of myogenic cells to the limb buds is an epitheliomesenchymal transformation of cells located at the lateral edge of the dermomyotome. Recently, a targeted mutation of c-met in mice demonstrated an essential role of this tyrosine kinase receptor and its ligand, scatter factor/hepatocyte growth factor (SF/HGF), in the migration of myogenic cells to the limb buds. Here, we show that ectopic application of exogenous SF/HGF induces emigration of Pax-3-positive myogenic cells into the lateral plate mesoderm. During this process, the lateral portions of the dermomyotomes deepithelialize and the basement membrane disintegrates. Detaching myogenic cells do not lose N-cadherin from their surfaces. We conclude that an HGF/SF- and c-met-mediated signal detaches myogenic precursor cells from the somites and thus plays a necessary role in the initiation of myoblast migration.
生肌细胞迁移至肢芽的起始事件是位于皮肌节外侧边缘的细胞发生上皮-间充质转化。最近，小鼠中c-met的靶向突变表明，这种酪氨酸激酶受体及其配体，即分散因子/肝细胞生长因子（SF/HGF），在生肌细胞向肢芽的迁移中起重要作用。在此，我们表明，外源性SF/HGF的异位应用可诱导Pax-3阳性生肌细胞迁移至侧板中胚层。在此过程中，皮肌节的外侧部分去上皮化，基底膜解体。分离的生肌细胞表面不会丢失N-钙黏着蛋白。我们得出结论，HGF/SF和c-met介导的信号使生肌前体细胞与体节分离，因此在成肌细胞迁移的起始过程中起必要作用。
