Copray S, Liem R, Mantingh-Otter I J, Brouwer N
Department of Medical Physiology, University of Groningen, The Netherlands.
Muscle Nerve. 1996 Nov;19(11):1401-12. doi: 10.1002/(SICI)1097-4598(199611)19:11<1401::AID-MUS4>3.0.CO;2-G.
With the aim to study the cellular mechanism underlying the process of muscle spindle regeneration, dorsal root ganglia (DRG) neurons derived from 16-day rat embryos were cocultured with developing myotubes in a compartmentalized culture device. To accomplish the selective survival and neurite formation of the proprioceptive subpopulation, the neurotrophic factor, neurotrophin-3, was added to the culture medium. It appeared that the proprioceptive DRG neurons could develop specialized, Ia afferent terminal-like contacts with myotubes. However, these interactions were scarce and did not result in the induction of differentiation of the contacted myotubes into intrafusal fibers as normally occurs during in vivo development. The present coculture setup apparently lacks appropriate regulatory factors essential for the proper matching of sensory axons and intrafusal fiber precursors and the induction of a functional sensory myoneural connection.
为了研究肌梭再生过程的细胞机制，将源自16天龄大鼠胚胎的背根神经节（DRG）神经元与发育中的肌管在分隔培养装置中共培养。为了实现本体感觉亚群的选择性存活和神经突形成，向培养基中添加了神经营养因子神经生长因子-3。结果表明，本体感觉DRG神经元可以与肌管形成特化的、Ia传入终末样接触。然而，这些相互作用很少，并且没有像在体内发育过程中正常发生的那样导致接触的肌管分化为梭内纤维。目前的共培养设置显然缺乏适当的调节因子，这些因子对于感觉轴突和梭内纤维前体的正确匹配以及功能性感觉肌神经连接的诱导至关重要。
