Morimoto M, Morita N, Ichikawa T, Kawata M
Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Japan.
Arch Histol Cytol. 1996 Aug;59(3):205-18. doi: 10.1679/aohc.59.205.
We assessed changes in neuropeptide Y (NPY), vasoactive intestinal peptide (VIP) and choline acetyltransferase (ChAT) immunoreactivities when neonatal rat chromaffin cells were cultured in a medium containing nerve growth factor (NGF), or the synthetic glucocorticoid dexamethasone (DEX), examining whether their expression was correlated with the morphological changes induced by NGF and DEX. All of the chromaffin cells in culture were tyrosine hydroxylase (TH)-immunopositive regardless of whether they extended processes. TH-immunoreactive materials of NGF-treated chromaffin cells were distributed in all the cytoplasmic processes, even at the tips of growth cones. The percentage of NPY-positive chromaffin cells did not change markedly when treated with NGF or DEX throughout the 14 days in culture. The proportion of VIP-positive chromaffin cells increased gradually in the NGF-treated group and that of ChAT-positive cells in the group was similar to VIP. The morphological alterations induced by NGF were not correlated with the changes in proportions of NPY-, VIP- or ChAT-positive chromaffin cells. The percentages of VIP- or ChAT-immunopositive chromaffin cells in the NGF-treated group showed much greater increases than those in the DEX-treated group. These findings suggest that NGF might modulate the phenotypic changes of neuropeptides and amines in rat chromaffin cells.
我们评估了新生大鼠嗜铬细胞在含有神经生长因子（NGF）或合成糖皮质激素地塞米松（DEX）的培养基中培养时神经肽Y（NPY）、血管活性肠肽（VIP）和胆碱乙酰转移酶（ChAT）免疫反应性的变化，以检查它们的表达是否与NGF和DEX诱导的形态变化相关。无论培养的嗜铬细胞是否伸出突起，所有细胞酪氨酸羟化酶（TH）免疫均呈阳性。NGF处理的嗜铬细胞的TH免疫反应物质分布于所有细胞质突起中，甚至在生长锥的尖端。在整个14天的培养过程中，用NGF或DEX处理时，NPY阳性嗜铬细胞的百分比没有明显变化。在NGF处理组中，VIP阳性嗜铬细胞的比例逐渐增加，且该组中ChAT阳性细胞的比例与VIP相似。NGF诱导的形态改变与NPY、VIP或ChAT阳性嗜铬细胞比例的变化无关。NGF处理组中VIP或ChAT免疫阳性嗜铬细胞的百分比增加幅度远大于DEX处理组。这些发现表明，NGF可能调节大鼠嗜铬细胞中神经肽和胺类的表型变化。
