Wilson W, Voigt P, Bader M, Marsden C A, Fink H
Department of Physiology and Pharmacology, Nottingham University, Queen's Medical Centre, UK.
Brain Res. 1996 Aug 5;729(1):1-9.
The first model of genetically engineered hypertension, the transgenic rat TGR (mREN2)27, provides a unique opportunity to study the behavioural effects of an altered brain renin-angiotensin system. The TGR (mREN2)27 rats, characterised by fulminant hypertension, show differences in both the peripheral and central angiotensin systems. The behaviour of male transgenic TGR (mREN2)27 and male Sprague-Dawley rats were determined by 4 behavioural tests. While on the elevated X-maze the TGR (mREN2)27 rat showed a greater 'anxiogenic' profile (fewer open arm entries) than the control Sprague-Dawley rats, this 'anxiogenic' profile increased further during a second exposure to the elevated X-maze 24 h later. In comparison the behaviour of the male Sprague-Dawley rats was not different between the two exposures to the elevated X-maze. Locomotor activity did not differ between either the TGR (mREN2)27 or Sprague-Dawley rats when placed in a 1 m2 open-field for 10 min. A short period of fluid-deprivation (3 h) reversed the 'anxiogenic' profile of the TGR (mREN2)27 on the elevated X-maze. Administration of captopril (20 mg . kg-1 body weight) in the drinking water of the TGR (mREN2)27 rats and Sprague-Dawley rats reversed the anxiogenic profile of the TGR (mREN2)27 rat on the elevated X-maze but did not alter the behaviour of the Sprague-Dawley rats.
首个基因工程高血压模型——转基因大鼠TGR (mREN2)27，为研究大脑肾素 - 血管紧张素系统改变的行为效应提供了独特契机。以暴发性高血压为特征的TGR (mREN2)27大鼠，在周围和中枢血管紧张素系统方面均表现出差异。通过4项行为测试确定了雄性转基因TGR (mREN2)27大鼠和雄性斯普拉格 - 道利大鼠的行为。在高架十字迷宫实验中，TGR (mREN2)27大鼠比对照的斯普拉格 - 道利大鼠表现出更强的“致焦虑”特征（进入开放臂的次数更少），而在24小时后再次暴露于高架十字迷宫时，这种“致焦虑”特征进一步增强。相比之下，雄性斯普拉格 - 道利大鼠在两次暴露于高架十字迷宫之间的行为没有差异。将TGR (mREN2)27大鼠和斯普拉格 - 道利大鼠置于1平方米的旷场中10分钟，二者的运动活动没有差异。短时间禁水（3小时）可逆转TGR (mREN2)27大鼠在高架十字迷宫上的“致焦虑”特征。给TGR (mREN2)27大鼠和斯普拉格 - 道利大鼠饮用含卡托普利（20毫克·千克体重）的水，可逆转TGR (mREN2)27大鼠在高架十字迷宫上的致焦虑特征，但不会改变斯普拉格 - 道利大鼠的行为。
