Gardino P F, Calaza K C, Hamassaki-Britto D E, Lindstrom J M, Britto L R, Hokoç J N
Department of Neurobiology, Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Brazil.
Brain Res Dev Brain Res. 1996 Sep 2;95(2):205-12. doi: 10.1016/0165-3806(96)00074-0.
Immunocytochemistry and [3H]thymidine autoradiography were combined in this study to determine the neurogenesis of cholinoceptive cells in the chick retina. After injections of [3H]thymidine between embryonic days 1 and 11, the time of birth of retinal neurons containing either the alpha 3 or the alpha 8 subunit of the nicotinic acetylcholine receptors was determined. The results indicate that the alpha 3-positive neurons in the ganglion cell layer leave the cell cycle from E2 through E7, and those in the inner nuclear layer (amacrine and displaced ganglion cells) from E2 through E9. The alpha 8-positive cells in the ganglion cell layer were born from E1 through E7, and those in the inner nuclear layer (amacrine and bipolar cells) from E2 through E11. These data suggest that the time of birth of cholinoceptive neurons in the chick retina follows the general pattern of cell generation in the chick retina, and that alpha 8-positive cells in the ganglion cell layer start to leave the cell cycle almost one day earlier than the alpha 3-positive cells in the same layer.
在本研究中，免疫细胞化学和[³H]胸腺嘧啶核苷放射自显影术相结合，以确定鸡视网膜中胆碱能感受细胞的神经发生。在胚胎第1天至第11天之间注射[³H]胸腺嘧啶核苷后，确定了含有烟碱型乙酰胆碱受体α3或α8亚基的视网膜神经元的诞生时间。结果表明，神经节细胞层中的α3阳性神经元在胚胎第2天至第7天离开细胞周期，内核层（无长突细胞和移位神经节细胞）中的α3阳性神经元在胚胎第2天至第9天离开细胞周期。神经节细胞层中的α8阳性细胞在胚胎第1天至第7天诞生，内核层（无长突细胞和双极细胞）中的α8阳性细胞在胚胎第2天至第11天诞生。这些数据表明，鸡视网膜中胆碱能感受神经元的诞生时间遵循鸡视网膜细胞生成的一般模式，并且神经节细胞层中的α8阳性细胞比同一层中的α3阳性细胞提前近一天开始离开细胞周期。
