Obermayr F, Walter M A, Jones H B, Goodfellow P N, Frischauf A M
Imperial Cancer Research Fund, London, UK.
Eur J Hum Genet. 1996;4(4):242-5. doi: 10.1159/000472206.
We have constructed a set of hybrid cell lines by irradiation of GM 64063 (human chromosome 9q only on hamster background) and fusion to hamster A23 Tk-, 109 independent lines were tested by PCR with 24 markers from chromosome 9q. The marker density is highest in the 9q22.3-q31 region containing the gene for Gorlin syndrome, a familial predisposition to basal cell carcinoma. The resolution of our map in this region is significantly higher than other published maps and will enable accurate placing of new markers and genes within the 9q22.3-q3.1 region. This is important since yeast artificial chromosomes from the region are likely to contain deletions.
我们通过对GM 64063（仅在仓鼠背景下含有人9号染色体q臂）进行辐射并与仓鼠A23 Tk-细胞融合，构建了一组杂交细胞系。用来自9号染色体q臂的24个标记对109个独立的细胞系进行了PCR检测。在包含戈林综合征（一种基底细胞癌的家族性易感性疾病）基因的9q22.3-q31区域，标记密度最高。我们在此区域的图谱分辨率明显高于其他已发表的图谱，这将有助于在9q22.3-q3.1区域内准确地定位新的标记和基因。这很重要，因为来自该区域的酵母人工染色体可能包含缺失。
