Licea A F, Becerril B, Possani L D
Department of Molecular Recognition and Structural Biology, National Autonomous University of Mexico, Cuernavaca, Mexico.
Toxicon. 1996 Aug;34(8):843-7. doi: 10.1016/0041-0101(96)00043-8.
BCF2 is a murine hybridoma cell line that produces a neutralizing antibody against toxin 2 (Cn2) from the scorpion Centruroides noxius Hoffmann of Mexico. In this communication we report the preparation and use of the BCF2 antibody and its antigen binding fragments (Fab) in experiments aiming at obtaining protection of experimental albino mice (strain CD1) challenged with purified toxin Cn2, as well as, with whole soluble venom from C. noxius. The monoclonal antibody BCF2 in amounts of 1 mg neutralizes 28 LD50 of soluble venom of C. noxius, whereas the Fab fragments of BCF2 (1 mg) are capable of neutralizing 43 LD50 dose of the same venom. To reach the same level of neutralization, with the commercially available horse antiserum [F(ab')2], we need to use about ninefold more material.
BCF2是一种小鼠杂交瘤细胞系，它能产生针对墨西哥毒蝎Centruroides noxius Hoffmann的毒素2（Cn2）的中和抗体。在本通讯中，我们报告了BCF2抗体及其抗原结合片段（Fab）的制备和使用情况，这些实验旨在保护用纯化毒素Cn2以及来自C. noxius的全可溶性毒液攻击的实验性白化小鼠（CD1品系）。1毫克的单克隆抗体BCF2可中和28个C. noxius可溶性毒液的半数致死量（LD50），而BCF2的Fab片段（1毫克）能够中和相同毒液43个LD50剂量。为了达到相同的中和水平，对于市售的马抗血清[F(ab')2]，我们需要使用大约九倍多的材料。
