Pesole G, Ceci L R, Gissi C, Saccone C, Quagliariello C
Dipartimento di Biologia D.B.A.F., Università della Basilicata, via Anzio 10, 85100 Potenza, Italy.
J Mol Evol. 1996 Nov;43(5):447-52.
We have analyzed the nad3-rps12 locus for eight angiosperms in order to compare the utility of mitochondrial DNA and edited mRNA sequences in phylogenetic reconstruction. The two coding regions, containing from 25 to 35 editing sites in the various plants, have been concatenated in order to increase the significance of the analysis. Differing from the corresponding chloroplast sequences, unedited mitochondrial DNA sequences seem to evolve under a quasi-neutral substitution process which undifferentiates the nucleotide substitution rates for the three codon positions. By using complete gene sequences (all codon positions) we found that genomic sequences provide a classical angiosperm phylogenetic tree with a clear-cut grouping of monocotyledons and dicotyledons with Magnoliidae at the basal branch of the tree. Conversely, owing to their low nucleotide substitution rates, edited mRNA sequences were found not to be suitable for studying phylogenetic relationships among angiosperms.
我们分析了8种被子植物的nad3 - rps12基因座，以比较线粒体DNA和编辑后的mRNA序列在系统发育重建中的效用。这两个编码区域在不同植物中含有25至35个编辑位点，已被拼接起来以提高分析的显著性。与相应的叶绿体序列不同，未编辑的线粒体DNA序列似乎在准中性替代过程中进化，该过程使三个密码子位置的核苷酸替代率没有差异。通过使用完整的基因序列（所有密码子位置），我们发现基因组序列提供了一个经典的被子植物系统发育树，单子叶植物和双子叶植物有明确的分组，木兰科位于树的基部分支。相反，由于其低核苷酸替代率，发现编辑后的mRNA序列不适合研究被子植物之间的系统发育关系。
