Dezawa M, Nagano T
Department of Anatomy, School of Medicine, Chiba University, Japan.
Anat Rec. 1996 Sep;246(1):114-26. doi: 10.1002/(SICI)1097-0185(199609)246:1<114::AID-AR13>3.0.CO;2-S.
The central nervous system neurons of adult mammals are known to regenerate into peripheral nerve autograft. The localization of cell adhesion molecules and cell-cell contact proteins were studied during axonal regeneration induced by sciatic nerve autotransplantation.
A sciatic nerve autograft was anastomosed to the proximal stump of the transected rat optic nerve. Immunofluorescence microscopy, thin sectioning, and immunoelectron microscopy with the preembedding method and ultrathin cryosections were used to localize cell adhesion molecules (L1; neural cell adhesion molecule, NCAM; myelin-associated glycoprotein, MAG) and cell-cell contact proteins (connexins 32, 43, ZO-1) at 3 days to 4 weeks postoperation.
Most regenerating axons contacted astrocytes in the optic nerve and Schwann cells in the graft. Immunoreactivity of NCAM was widely distributed along the surface of axons, astrocytes, Schwann cells, and perineurial cells. The L1 immunoreactivity was confined to the interface of axon-astrocyte and of axon-Schwann cell. MAG immunoreactivity was seen at the interface of axon and myelin within the graft. Connexins 32, 43, and ZO-1 immunoreactivities were observed at contact sites between axons and Schwann cells within the graft.
Cell adhesion molecules (L1, NCAM, MAG) are localized at the cell surface of regenerating axons, astrocytes, and Schwann cells during optic nerve regeneration elicited by peripheral nerve graft. Cell-cell contact proteins (connexins 32, 43, ZO-1) are present at the interface between axons and Schwann cells in the graft. Our results suggest that these molecules are involved in cell adhesion events during optic nerve regeneration.
已知成年哺乳动物的中枢神经系统神经元可再生进入周围神经自体移植体。在坐骨神经自体移植诱导的轴突再生过程中，对细胞黏附分子和细胞间接触蛋白的定位进行了研究。
将坐骨神经自体移植体吻合至切断的大鼠视神经近端残端。采用免疫荧光显微镜、薄切片以及预包埋法免疫电子显微镜和超薄冰冻切片，在术后3天至4周定位细胞黏附分子（L1；神经细胞黏附分子，NCAM；髓鞘相关糖蛋白，MAG）和细胞间接触蛋白（连接蛋白32、43、紧密连接蛋白1，ZO-1）。
大多数再生轴突与视神经中的星形胶质细胞以及移植体中的施万细胞接触。NCAM的免疫反应性广泛分布于轴突、星形胶质细胞、施万细胞和神经束膜细胞表面。L1免疫反应性局限于轴突 - 星形胶质细胞和轴突 - 施万细胞的界面。在移植体内轴突与髓鞘的界面可见MAG免疫反应性。在移植体内轴突与施万细胞的接触部位观察到连接蛋白32、43和紧密连接蛋白1的免疫反应性。
在周围神经移植引发的视神经再生过程中，细胞黏附分子（L1、NCAM、MAG）定位于再生轴突、星形胶质细胞和施万细胞的细胞表面。细胞间接触蛋白（连接蛋白32、43、紧密连接蛋白1）存在于移植体中轴突与施万细胞的界面。我们的结果表明，这些分子参与了视神经再生过程中的细胞黏附事件。
