Lin C, Hsieh J Y, Matuszewski B K, Dobrinska M R
Department of Drug Metabolism, Merck Research Laboratories, West Point, PA 19486, USA.
J Pharm Biomed Anal. 1996 Aug;14(11):1601-8. doi: 10.1016/0731-7085(96)01737-2.
A high performance liquid chromatography assay utilizing an automated sample preparation procedure for the determination of a novel leukotriene biosynthesis inhibitor, (MK-591), in human plasma has been developed. After aliquoting plasma samples and adding internal standard manually, the BenchMate Workstation executed protein precipitation and solid-phase extraction. Following evaporation to dryness, the residue was reconstituted and chromatographed isocratically on a cyano-phase analytical column. MK-591 and the internal standard were separated from each other and from endogenous plasma substances and detected with an absorbance detector. The assay has been validated in the concentration range 10-1000 ng ml-1 and has the sensitivity and specificity necessary to quantify plasma concentrations from several clinical studies.
已开发出一种高效液相色谱分析方法，该方法利用自动样品制备程序测定人血浆中一种新型白三烯生物合成抑制剂（MK-591）。在手动等分血浆样品并加入内标后，BenchMate工作站执行蛋白质沉淀和固相萃取。蒸发至干后，将残渣复溶，并在氰基相分析柱上进行等度色谱分析。MK-591和内标彼此分离，并与内源性血浆物质分离，然后用吸光度检测器进行检测。该分析方法在10 - 1000 ng ml-1的浓度范围内得到验证，具有量化多项临床研究中血浆浓度所需的灵敏度和特异性。
