Bugajski J, Gadek-Michalska A, Borycz J, Głód R, Ołowska A
Department of Physiology, Polish Academy of Sciences, Cracow, Poland.
J Physiol Pharmacol. 1996 Sep;47(3):487-95.
Involvement of prostaglandins (PGs) and histamine in the hypothalamus and hippocampus in the clonidine-induced pituitary-adrenocortical response was investigated in conscious rats. The hypothalamic-pituitary adrenocortical (HPA) activity was assessed indirectly by measuring corticosterone secretion. Clonidine, an alpha 2-adrenergic agonist, given intracerebroventricularly (10 micrograms icv), considerably increased the serum corticosterone and hypothalamic histamine levels and markedly elevated the hippocampal histamine levels. Systemic or icv pretreatment with indomethacin (2 mg/kg or 10 micrograms), an inhibitor of prostaglandin synthesis, significantly reduced the clonidine-induced corticosterone response and abolished the increase in the hypothalamic and hippocampal histamine levels elicited by clonidine. Indomethacin in the doses used did not substantially change the resting serum corticosterone or hypothalamic and hippocampal histamine levels. These results indicate that prostaglandins and hypothalamic histamine are considerably involved in the HPA response to alpha 2-adrenergic receptor stimulation. They also suggest involvement of prostaglandins and histamine of the hippocampus in the clonidine-induced HPA response.
在清醒大鼠中，研究了前列腺素（PGs）和组胺在下丘脑和海马体中参与可乐定诱导的垂体 - 肾上腺皮质反应的情况。通过测量皮质酮分泌间接评估下丘脑 - 垂体 - 肾上腺皮质（HPA）活性。α2 - 肾上腺素能激动剂可乐定经脑室内注射（10微克/脑室内）后，显著增加血清皮质酮和下丘脑组胺水平，并明显提高海马体组胺水平。用前列腺素合成抑制剂吲哚美辛（2毫克/千克或10微克）进行全身或脑室内预处理，可显著降低可乐定诱导的皮质酮反应，并消除可乐定引起的下丘脑和海马体组胺水平升高。所用剂量的吲哚美辛并未显著改变静息状态下的血清皮质酮或下丘脑及海马体组胺水平。这些结果表明，前列腺素和下丘脑组胺在HPA对α2 - 肾上腺素能受体刺激的反应中起重要作用。它们还提示海马体中的前列腺素和组胺参与了可乐定诱导的HPA反应。
