Cheung A N, Tin V P, Ngan H Y, Chung L P, Khoo U S
Department of Pathology, University of Hong Kong, Queen Mary Hospital, Hong Kong.
Mod Pathol. 1996 Sep;9(9):910-8.
Endometrial stromal neoplasms include a heterogenous group of tumors with different clinical behavior and response to treatment. Cytogenetic study of such tumors has been scanty. The aim of this study was to analyze and compare the chromosome composition in low-grade and high-grade endometrial stromal sarcoma using the technique of chromosome in situ hybridization. Eight cases of low-grade stromal sarcoma and three cases of high-grade stromal sarcoma were studied. Biotinylated DNA probes specific for the regions of chromosomes X, 11, 12, and 17 were used on formalin-fixed paraffin embedded material from these tumors. The in situ hybridization signals were visualized by immunoperoxidase technique. Four of the eight low-grade stromal sarcomas retained normal disomy of the chromosomes studied. The other four low-grade sarcomas showed a gain of from one to three chromosomes, whereas all of the three high-grade sarcomas showed polysomies in all of the four chromosomes being studied. No loss of chromosomes was detected. One case of high-grade sarcoma contained coexisting areas of low-grade sarcoma. Although aneusomy was found in the high-grade portion, disomy was noted in the low-grade areas. In the stromal sarcomas studied, there was no definite correlation between the presence of chromosome polysomies and the clinical progress of the tumors. This is the first interphase cytogenetic study of uterine stromal sarcoma, and the results support the concept that complex numerical chromosome abnormalities evolve during anaplastic transformation of endometrial stromal sarcoma.
子宫内膜间质肿瘤包括一组具有不同临床行为和治疗反应的异质性肿瘤。对这类肿瘤的细胞遗传学研究一直很少。本研究的目的是使用染色体原位杂交技术分析和比较低级别和高级别子宫内膜间质肉瘤的染色体组成。研究了8例低级别间质肉瘤和3例高级别间质肉瘤。对这些肿瘤的福尔马林固定石蜡包埋材料使用了针对X、11、12和17号染色体区域的生物素化DNA探针。通过免疫过氧化物酶技术观察原位杂交信号。8例低级别间质肉瘤中有4例在所研究的染色体上保持正常二倍体。其他4例低级别肉瘤显示有1至3条染色体的增加，而3例高级别肉瘤在所研究的所有4条染色体上均显示多体性。未检测到染色体缺失。1例高级别肉瘤包含共存的低级别肉瘤区域。虽然在高级别部分发现了非整倍体，但在低级别区域观察到二倍体。在所研究的间质肉瘤中，染色体多体性的存在与肿瘤的临床进展之间没有明确的相关性。这是首次对子宫间质肉瘤进行的间期细胞遗传学研究，结果支持这样的概念，即复杂的染色体数目异常在子宫内膜间质肉瘤的间变转化过程中演变。
