Li J L, Kaneko T, Mizuno N
Department of Morphological Brain Science, Faculty of Medicine, Kyoto University, Japan.
Neurosci Lett. 1996 Aug 23;214(2-3):91-4. doi: 10.1016/0304-3940(96)12894-9.
The present study was attempted to examine if mu-opioid receptor (MOR) might be transported by axonal flow peripherally through peripheral axons of somatic sensory ganglion neurons. After unilateral ligation of the sciatic nerve or the vagus nerve distal to the dorsal root ganglion (DRG) or nodose ganglion (NG), MOR-like immunoreactivity (MOR-LI) of neuronal cell bodies in the DRG of the fourth and fifth lumbar nerves, NG, ambiguus nucleus (Amb) and dorsal motor nucleus of the vagus nerve (DMV) on the side of the ligation was apparently reduced within 1 week after the nerve ligation. However, within 24 h after the nerve ligation, a transient enhancement of MOR-LI was observed in cell bodies of DRG neurons, sciatic nerve stump proximal to the ligature, and cell bodies of NG neurons on the side of the ligation; such a transient enhancement of MOR-LI was not detected in the Amb and DMV. The results suggest that MOR undergoes centrifugal axonal flow in peripheral axons of somatic and visceral sensory ganglion neurons, and that MOR synthesis in sensory ganglion neurons is vulnerable to damage of the peripheral axons.
本研究旨在探讨μ-阿片受体（MOR）是否可能通过轴浆流沿躯体感觉神经节神经元的外周轴突向周围运输。在背根神经节（DRG）或结状神经节（NG）远端单侧结扎坐骨神经或迷走神经后，结扎侧第四和第五腰神经DRG、NG、疑核（Amb）和迷走神经背运动核（DMV）中神经元胞体的MOR样免疫反应性（MOR-LI）在神经结扎后1周内明显降低。然而，在神经结扎后24小时内，在结扎侧DRG神经元的胞体、结扎近端的坐骨神经残端以及NG神经元的胞体中观察到MOR-LI的短暂增强；在Amb和DMV中未检测到这种MOR-LI的短暂增强。结果表明，MOR在躯体和内脏感觉神经节神经元的外周轴突中经历离心轴浆流，并且感觉神经节神经元中的MOR合成易受外周轴突损伤的影响。
