Sugiyama T, Suzue K, Okamoto M, Inselburg J, Tai K, Horii T
Department of Molecular Protozoology, Osaka University, Japan.
Vaccine. 1996 Aug;14(11):1069-76. doi: 10.1016/0264-410x(95)00238-v.
We expressed two regions of the serine repeat antigen (SERA) protein of Plasmodium falciparum in Escherichia coli by synthesizing the genes with a changed codon usage. One of the synthetic gene sequences encodes amino acid residues 17-382 (SE47') and the other encodes amino acid residues 586-802 (SE50A). The products produced by the synthetic gene sequences in E. coli accounted for 15-30% of the total bacterial protein. Antisera against both the purified gene products prepared in rats inhibited malaria parasite growth in vitro. The anti-SE47' serum was significantly more inhibitory than the anti-SE50A serum. The described methods provide a large scale preparation of recombinant antigens for improving and producing malaria vaccine.
我们通过合成密码子使用改变的基因，在大肠杆菌中表达了恶性疟原虫丝氨酸重复抗原（SERA）蛋白的两个区域。其中一个合成基因序列编码氨基酸残基17 - 382（SE47'），另一个编码氨基酸残基586 - 802（SE50A）。合成基因序列在大肠杆菌中产生的产物占细菌总蛋白的15% - 30%。用大鼠制备的针对两种纯化基因产物的抗血清在体外抑制疟原虫生长。抗SE47'血清的抑制作用明显强于抗SE50A血清。所描述的方法为改进和生产疟疾疫苗提供了大规模制备重组抗原的途径。
