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[通过转染在人类肿瘤DNA中寻找病毒遗传信息]

[Search for viral genetic information in human tumor DNA by means of transfection].

作者信息

Kniazev P G, Perevozchikov A P, Korobitsyn L P, Zhudina A I, Kuznetsov O K

出版信息

Vopr Onkol. 1979;25(5):50-5.

PMID:88806
Abstract

In embryonal human cell culture there was found the biological activity of DNA preparations, obtained from peripheral blood cells of acute leukemia patients, which was manifested in the production of virus-like RNA-containing particles, as evidenced by the radioisotope analysis findings and by the reverse transcriptase content in these particles. Also, a rapid growth of the cultures was noted. The DNA preparations from rhabdomyosarcoma, neurinoma and synovial sarcoma in man would cause only an enhancement of human embryonal cell cultures growth. No production of virus-like particles in the latter was noted.

摘要

在人胚胎细胞培养中,发现从急性白血病患者外周血细胞获得的DNA制剂具有生物活性,这表现为产生含病毒样RNA的颗粒,放射性同位素分析结果以及这些颗粒中的逆转录酶含量证明了这一点。此外,还注意到培养物的快速生长。来自人横纹肌肉瘤、神经瘤和滑膜肉瘤的DNA制剂只会促进人胚胎细胞培养物的生长。在后者中未发现产生病毒样颗粒。

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