Fischer U, Meltzer P, Meese E
Department of Human Genetics, Medical School, University Hospital, University of Saar, Homburg/Saar, Germany.
Hum Genet. 1996 Nov;98(5):625-8. doi: 10.1007/s004390050271.
Gene amplification has been associated both with tumor stage and progression in human gliomas. Several distinct amplified loci have been identified by comparative genomic hybridization and Southern blot analysis. It has been increasingly recognized that amplified domains comprise multiple genes. Here, we demonstrate amplification of up to 12 different genes from an amplified domain at 12q13-15 that has been found in approximately 15% of astrocytomas and glioblastomas. The amplified genes were GLI, WNT1, MDM2, SAS, CDK4 OS-4, GAS16, GAS27, GAS41, GAS56, GAS 64 and GAS89. In one glioblastoma all 12 amplified genes were also found to be expressed. These results strongly warrant the search for as yet unidentified genes in regions previously reported to be amplified.
基因扩增与人类胶质瘤的肿瘤分期和进展均相关。通过比较基因组杂交和Southern印迹分析已鉴定出几个不同的扩增位点。人们越来越认识到扩增区域包含多个基因。在此，我们证明了在12q13 - 15的一个扩增区域中多达12个不同基因的扩增，该区域在大约15%的星形细胞瘤和胶质母细胞瘤中被发现。扩增的基因有GLI、WNT1、MDM2、SAS、CDK4 OS - 4、GAS16、GAS27、GAS41、GAS56、GAS 64和GAS89。在一个胶质母细胞瘤中还发现所有12个扩增基因均有表达。这些结果有力地支持了在先前报道为扩增的区域中寻找尚未鉴定的基因。
