Kühn A G, Arnold W
HNO-Klinik und Poliklinik der Technischen Universität, Klinikum rechts der Isar, München, Deutschland.
ORL J Otorhinolaryngol Relat Spec. 1996 Jul-Aug;58(4):229-32. doi: 10.1159/000276842.
The presence of neuropeptide-containing sensory, parasympathetic and sympathetic nerves in human nasal mucosa is well established. However, information regarding the neuropeptide distribution in nasal polyps is still lacking. The aim of this study was to compare immunohistologically the presence and distribution of substance P (SP), vasoactive intestinal peptide (VIP) and bombesin-flanking peptide (BFP) in normal nasal mucosa and nasal polyps. The expression was studied using Formalin-fixed and paraffin-embedded biopsy sections from 20 patients with nasal polyps and 10 controls with normal nasal mucosa from the anterior part of the inferior turbinate. Indirect immunohistochemistry (APAAP method) with mono- and polyclonal antibodies was used. None of the control group but 5 patients of the nasal polyp group had an atopic disease. Comparing localization and intensity of the immunohistological reaction in nasal mucosa and nasal polyp tissue, there was no difference in the expression of the three neuroendocrine substances. There was no difference of expression in the polyposis group itself concerning allergic and nonallergic subjects. The results indicate that VIP, SP and BFP have an equal distribution in nasal polyps and normal nasal mucosa.
人体鼻黏膜中存在含神经肽的感觉神经、副交感神经和交感神经，这一点已得到充分证实。然而，关于神经肽在鼻息肉中的分布情况仍缺乏相关信息。本研究的目的是通过免疫组织化学方法比较正常鼻黏膜和鼻息肉中P物质（SP）、血管活性肠肽（VIP）和蛙皮素侧翼肽（BFP）的存在情况及分布。使用来自20例鼻息肉患者和10例下鼻甲前部正常鼻黏膜对照者的福尔马林固定石蜡包埋活检切片进行表达研究。采用单克隆和多克隆抗体进行间接免疫组织化学（APAAP法）。对照组中无人患有特应性疾病，而鼻息肉组中有5例患者患有特应性疾病。比较鼻黏膜和鼻息肉组织中免疫组织化学反应的定位和强度，三种神经内分泌物质的表达无差异。在息肉病组中，过敏和非过敏受试者之间的表达也无差异。结果表明，VIP、SP和BFP在鼻息肉和正常鼻黏膜中的分布相同。
