Hidaka S, Nishimura H, Nakajima K, Liu S Y
Department of Oral Biochemistry, Fukuoka Dental College, Tokyo, Japan.
J Periodontal Res. 1996 Aug;31(6):408-13. doi: 10.1111/j.1600-0765.1996.tb00509.x.
Inhibitory effects of a rhubarb (Rhei rhizoma) solution and its fractions on the formation of calcium phosphate precipitates were studied. The rhubarb solution inhibited both the amorphous calcium phosphate (ACP) formation and the rate of hydroxyapatite (HAP) transformation, and extended the induction time. When the solution was fractionated using membrane filters, a filtrate with the molecular weight between 3 and 10 kDa (with 2/3 recovery of polyphenols) was found to be responsible for both the ACP formation and the extension of the induction time. Another filtrate with the molecular weight below 3 kDa (with 1/3 recovery of polyphenols) may be responsible for the inhibition of both the ACP formation and the rate of HAP transformation, and the extension of the induction time. When the extract of rhubarb was fractionated using a Sephadex LH-20 column chromatography, fraction IV greatly inhibited the formation of calcium phosphate precipitates, while fractions I, II and III slightly inhibited that reaction. Our finding suggests that fraction IV may contain useful substance(s) for the prevention of oral calcium phosphate precipitation (calculus formation). However, strong calcium chelating properties would limit the concentration that could be safely employed.
研究了大黄（Rhei rhizoma）溶液及其组分对磷酸钙沉淀形成的抑制作用。大黄溶液既抑制了无定形磷酸钙（ACP）的形成，又抑制了羟基磷灰石（HAP）的转化速率，并延长了诱导时间。当使用膜过滤器对溶液进行分级分离时，发现分子量在3至10 kDa之间的滤液（多酚回收率为2/3）对ACP的形成和诱导时间的延长均有作用。另一种分子量低于3 kDa的滤液（多酚回收率为1/3）可能对ACP形成和HAP转化速率的抑制以及诱导时间的延长都有作用。当使用葡聚糖LH-20柱色谱对大黄提取物进行分级分离时，组分IV对磷酸钙沉淀的形成有很大抑制作用，而组分I、II和III对该反应有轻微抑制作用。我们的研究结果表明，组分IV可能含有预防口腔磷酸钙沉淀（牙结石形成）的有用物质。然而，强钙螯合特性会限制其安全使用的浓度。
