Brog J S, Zahm D S
Department of Anatomy and Neurobiology, St Louis University School of Medicine, MO 63104, USA.
Neuroscience. 1996 Oct;74(3):805-12. doi: 10.1016/0306-4522(96)00166-2.
It has been reported in previous studies that perikaryal neurotensin immunoreactivity is largely absent in the rat striatum except following striatal dopamine depletion or blockade of dopamine D2 receptors, after which, however, neurotensin immunoreactivity is elicited in at least two distinct subpopulations of striatal neurons [Zahm D.S. (1992) Neuroscience 46, 335-350]. One subpopulation of such cells (type I), prominent following D2 receptor blockade, is located mainly in the matrix compartment in the rostral, dorsomedial and ventrolateral parts of the striatum, and comprises neurons at the large end of the medium-sized spectrum that exhibit intense neurotensin immunoreactivity in perikarya and proximal dendrites, but rarely display Fos immunoreactivity [Senger B. et al. (1993) Neuroscience 57, 649-660]. A second subpopulation (type II) resides predominantly in the patch (striosome) and matrix compartments in the dorsolateral quadrant of the striatum, and is prominent following administration of reserpine. These neurons are at the small end of the medium size range and exhibit very light neurotensin immunoreactivity, with little staining of dendrites. Fos immunoreactivity is frequently co-localized in striatal neurons that exhibit a type II striatal neurotensin response [Brog J.S. and Zahm D.S. (1995) Neuroscience 65, 71-86]. In the current study, neurotensin immunoreactivity was elicited in striatal neurons by ventral mesencephalic 6-hydroxydopamine lesions or administration of reserpine or haloperidol. Irrespective of which drug was given, retrogradely transported Fluoro-Gold was prominently co-localized with neurotensin-like immunofluorescence in the perikarya of striatal neurons following injections of the retrograde tracer into the globus pallidus. Few double-labeled neurons were observed following administration of any of these drugs and injections of Fluoro-Gold into the substantia nigra. It is concluded that two subpopulations of neurotensin-immunoreactive striatal neurons project predominantly to the globus pallidus and minimally to the substantia nigra.
先前的研究报道，除了纹状体多巴胺耗竭或多巴胺D2受体阻断后，大鼠纹状体中核周神经降压素免疫反应性在很大程度上不存在，然而，在此之后，至少在两个不同的纹状体神经元亚群中引发了神经降压素免疫反应性[扎姆D.S.（1992年）《神经科学》46卷，335 - 350页]。这类细胞的一个亚群（I型），在D2受体阻断后很突出，主要位于纹状体前部、背内侧和腹外侧部分的基质区，由中等大小范围较大端的神经元组成，这些神经元在核周体和近端树突中表现出强烈的神经降压素免疫反应性，但很少显示Fos免疫反应性[森格尔B.等人（1993年）《神经科学》57卷，649 - 660页]。第二个亚群（II型）主要位于纹状体背外侧象限的斑块（纹状体小体）和基质区，在给予利血平后很突出。这些神经元处于中等大小范围的小端，表现出非常淡的神经降压素免疫反应性，树突几乎没有染色。Fos免疫反应性经常共定位于表现出II型纹状体神经降压素反应的纹状体神经元中[布罗格J.S.和扎姆D.S.（1995年）《神经科学》65卷，71 - 86页]。在当前研究中，通过腹侧中脑6 - 羟基多巴胺损伤或给予利血平或氟哌啶醇在纹状体神经元中引发神经降压素免疫反应性。无论给予哪种药物，在将逆行示踪剂注入苍白球后，逆行运输的荧光金在纹状体神经元的核周体中与神经降压素样免疫荧光显著共定位。在给予这些药物中的任何一种并将荧光金注入黑质后，观察到的双标记神经元很少。得出的结论是，神经降压素免疫反应性纹状体神经元的两个亚群主要投射到苍白球，而极少投射到黑质。
