Yasuda H, Murayama M, Yamamoto O, Asahi M
Department of Dermatology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Scand J Plast Reconstr Surg Hand Surg. 1996 Sep;30(3):169-75. doi: 10.3109/02844319609062810.
Isologous free skin grafts were applied to the backs of BALB/c mice and contact hypersensitivity studied by epicutaneous application of DNFB (2,4-dinitro-1-fluorobenzene) to the grafted skin. In the first experiment, the grafted skin was sensitised and the elicitation reaction assessed by measuring the ear swelling after five days. Sensitisation was not successful until two weeks after grafting. In such non-responding animals, re-sensitisation with DNFB on the ungrafted skin area was also unsuccessful, indicating the establishment of immunological tolerance. This tolerance was considered antigen-specific, as re-sensitisation with oxazolone was successful. In the second experiment, spleen cell suspension, both untreated and treated in vitro with antiThy 1.2, antiLyt 1.2, and antiLyt 2.2 antibody, from non-DNFB-responding mice was transferred into normal mice. Subsequently these mice were sensitised with DNFB. Sensitisation was not successful in the untreated group or in those treated with antiLyt 1.2 antibody. On the other hand, the groups treated with antiThy 1.2 and antiLyt 2.2 antibody did become sensitised. These results indicate that the unsuccessful delayed hypersensitivity of the grafted skin was caused by suppressor T cells. In addition, the density of epidermal Langerhans cells was reduced in the early stages of skin grafting and morphological abnormalities were present. From these results, we conclude that contact sensitisation during the early stage of grafting skin not only produces suppression of ear swelling but also induces antigen-specific tolerance. These results also suggest that the suppression depends on the antigen-specific suppressor cells and that acquirement of tolerance is associated with a reduction in the number of epidermal Langerhans cells in the grafted skin and abnormalities in their structure.
将同种异体游离皮片移植到BALB/c小鼠背部，并通过在移植皮肤上经皮涂抹二硝基氟苯（DNFB）来研究接触性超敏反应。在第一个实验中，对移植皮肤进行致敏，并在五天后通过测量耳部肿胀来评估激发反应。直到移植后两周才成功致敏。在这些无反应的动物中，在未移植皮肤区域用DNFB再次致敏也未成功，这表明建立了免疫耐受。这种耐受被认为是抗原特异性的，因为用恶唑酮再次致敏是成功的。在第二个实验中，将来自对DNFB无反应小鼠的脾细胞悬液，分别未经处理以及在体外经抗Thy 1.2、抗Lyt 1.2和抗Lyt 2.2抗体处理后，转移到正常小鼠体内。随后，用DNFB对这些小鼠进行致敏。未处理组或用抗Lyt 1.2抗体处理的组致敏未成功。另一方面，用抗Thy 1.2和抗Lyt 2.2抗体处理的组确实发生了致敏。这些结果表明，移植皮肤延迟性超敏反应未成功是由抑制性T细胞引起的。此外，在皮肤移植早期，表皮朗格汉斯细胞的密度降低，并且存在形态学异常。从这些结果中，我们得出结论，在移植皮肤早期的接触致敏不仅会抑制耳部肿胀，还会诱导抗原特异性耐受。这些结果还表明，这种抑制取决于抗原特异性抑制细胞，并且耐受的获得与移植皮肤中表皮朗格汉斯细胞数量的减少及其结构异常有关。
