大鼠RAB 3C GTP结合蛋白的克隆与组织表达

Cloning and tissue expression of the rat RAB 3C GTP-binding protein.

作者信息

Viggeswarapu M, Wildey G M

机构信息

Department of Molecular Cardiology, Cleveland Clinic Foundation, Ohio 44195, USA.

出版信息

Biochem Biophys Res Commun. 1996 Oct 23;227(3):645-50. doi: 10.1006/bbrc.1996.1563.

DOI:10.1006/bbrc.1996.1563

PMID:8885988

Abstract

A cDNA clone which encodes the low molecular weight GTP-binding protein rab 3C was isolated from a rat PC12 pheochromocytoma library. The 1.0 kb clone contains the entire coding region (681 bp), as well as 5' (89 bp) and 3' (230 bp) untranslated sequences. The predicted amino acid sequence of the rat rab 3C clone is 98% identical to the bovine rab 3C sequence and 85% identical to the rat rab 3A sequence. Northern blot analyses using probes containing coding and noncoding sequences of the rat rab 3C clone hybridized to a 9.5 kb transcript in brain, adrenal gland, and pituitary RNA pools.

摘要

从大鼠嗜铬细胞瘤PC12文库中分离出一个编码低分子量GTP结合蛋白rab 3C的cDNA克隆。这个1.0 kb的克隆包含完整的编码区（681 bp）以及5'非翻译序列（89 bp）和3'非翻译序列（230 bp）。大鼠rab 3C克隆的预测氨基酸序列与牛rab 3C序列的同源性为98%，与大鼠rab 3A序列的同源性为85%。使用包含大鼠rab 3C克隆编码和非编码序列的探针进行的Northern印迹分析显示，该探针与脑、肾上腺和垂体RNA样本中的一个9.5 kb转录本杂交。