Mertsalov I B, Gundelfinger E, Tsetlin V I
Institute of Neurophysiology, Magdeburg, Germany.
Bioorg Khim. 1996 May;22(5):366-9.
By using the human fetal brain library, a 1.2-kb cDNA coding for human neurogranin, a neurospecific endogenous substrate of protein kinase C, was cloned. The results of the Northern blot analysis of the human brain cortex mRNA show that the human neurogranin mRNA exists as a single transcript. Its gene thereby differs from the rat neurogranin gene (RC3), which was shown to produce two mRNAs of different lengths. cDNAs of the human and rat neurogranins show a 70% homology, whereas the protein sequences only differ in three amino acid residues out of the total of 78. The 3'-untranslated region of the human neurogranin cDNA lacks the purine-rich stretch characteristic of the rat neurogranin cDNA.
通过使用人类胎儿脑文库，编码人神经颗粒蛋白（一种蛋白激酶C的神经特异性内源性底物）的1.2 kb cDNA被克隆出来。对人类大脑皮质mRNA进行Northern印迹分析的结果表明，人神经颗粒蛋白mRNA以单一转录本形式存在。因此，其基因与大鼠神经颗粒蛋白基因（RC3）不同，后者可产生两种长度不同的mRNA。人和大鼠神经颗粒蛋白的cDNA显示出70%的同源性，而蛋白质序列在总共78个氨基酸残基中仅在三个氨基酸残基上有所不同。人神经颗粒蛋白cDNA的3'非翻译区缺乏大鼠神经颗粒蛋白cDNA特有的富含嘌呤的片段。
