Miura H, Tomoda H, Miura K, Takishima K, Omura S
Research Center for Biological Function, Kitasato Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1996 Oct 23;227(3):684-7. doi: 10.1006/bbrc.1996.1569.
Low density lipoprotein (LDL) receptor expression is regulated by proteolysis of transcription factors, sterol regulatory element binding proteins. To investigate the involvement of proteasome in this regulatory process, the effect of lactacystin, a specific proteasome inhibiton of microbial origin, was tested on LDL receptor expression on HepG2 cells. The addition of lactacystin to the cell culture increased the number of LDL receptors, indicating that proteasome plays a down-regulatory role in LDL receptor expression.
低密度脂蛋白（LDL）受体的表达受转录因子——固醇调节元件结合蛋白的蛋白水解作用调控。为了研究蛋白酶体在这一调控过程中的作用，我们检测了源于微生物的特异性蛋白酶体抑制剂——乳胞素对HepG2细胞上LDL受体表达的影响。向细胞培养物中添加乳胞素会增加LDL受体的数量，这表明蛋白酶体在LDL受体表达中起下调作用。
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