Tolou H, Puggelli H, Tock F, Durand J P
IMTSSA, Laboratoire de Biologie Moléculaire des Virus, Marseille Armées, France.
Acta Virol. 1996 Apr;40(2):73-9.
Phosphorothioate oligodeoxyribonucleotides targeted to different regions of the viral genome were synthesized and used in two kinds of experiments testing their activity against yellow fever virus (YFV) replication in cultured cells. We found that oligonucleotides complementary to the 3'-end or to the coding region of the viral RNA were regularly active in plaque reduction assay, although with inconstant efficiency. Oligonucleotides targeted to the 5'-end or to the initiation codon region exhibited lesser activity. Homologous oligonucleotides targeted to dengue virus RNA had no detectable inhibitory activity against dengue virus replication. However, in YFV production reduction assay, a non-specific inhibitory activity of a random oligonucleotide was observed. Taken as a whole, our results indicate that flaviviruses present detectable but heterogeneous sensitivity to phosphorothioate inhibition. Possible explanations are discussed.
合成了靶向病毒基因组不同区域的硫代磷酸酯寡脱氧核糖核苷酸，并将其用于两类实验，以测试它们对培养细胞中黄热病毒（YFV）复制的活性。我们发现，与病毒RNA的3'-末端或编码区互补的寡核苷酸在蚀斑减少试验中通常具有活性，尽管效率不稳定。靶向5'-末端或起始密码子区域的寡核苷酸活性较低。靶向登革病毒RNA的同源寡核苷酸对登革病毒复制没有可检测到的抑制活性。然而，在YFV产生减少试验中，观察到一种随机寡核苷酸的非特异性抑制活性。总体而言，我们的结果表明黄病毒对硫代磷酸酯抑制表现出可检测但异质性的敏感性。文中讨论了可能的解释。
