Kato K, Yang H, Taché Y
CURE/Digestive Disease Research Center, West Los Angeles VA Medical Center, CA 90073, USA.
Digestion. 1996;57(5):322-7. doi: 10.1159/000201352.
The influence of central vagal activation induced by the thyrotropin-releasing hormone (TRH) analog, RX 77368, injected intracisternally on gastric mucosal injury produced by 60% ethanol (4 ml.kg-1) was investigated in urethane-anesthetized rats. RX 77368 (3, 10 and 30 ng) inhibits dose dependently macroscopic gastric damage induced by intragastric administration of 60% ethanol by 18, 43 and 77%, respectively. The cytoprotective effect of intracisternal RX 77368 (30 ng) was completely blocked by cervical vagotomy, atropine (2 mg.kg-1 s.c.), and CGRP8-37 (100 micrograms.kg-1 i.v.) and partially inhibited by indomethacin (5 mg.kg-1 i.p.). Vagotomy, atropine, indomethacin and CGRP8-37 alone did not modify gastric mucosal lesions induced by ethanol in rats injected intracisternally with saline. The present data show that intracisternal injection of TRH analog in urethane-anesthetized rats protects against ethanol-induced gastric injury through vagal cholinergic dependent pathways which recruit prostaglandins and CGRP mechanisms.
在乌拉坦麻醉的大鼠中，研究了脑池内注射促甲状腺激素释放激素（TRH）类似物RX 77368诱导的中枢迷走神经激活对60%乙醇（4 ml·kg-1）所致胃黏膜损伤的影响。RX 77368（3、10和30 ng）分别剂量依赖性地抑制胃内给予60%乙醇诱导的宏观胃损伤18%、43%和77%。脑池内注射RX 77368（30 ng）的细胞保护作用被颈迷走神经切断术、阿托品（2 mg·kg-1皮下注射）和CGRP8-37（100 μg·kg-1静脉注射）完全阻断，被吲哚美辛（5 mg·kg-1腹腔注射）部分抑制。单独的迷走神经切断术、阿托品、吲哚美辛和CGRP8-37对脑池内注射生理盐水的大鼠乙醇诱导的胃黏膜损伤没有影响。目前的数据表明，在乌拉坦麻醉大鼠中脑池内注射TRH类似物可通过迷走胆碱能依赖性途径保护免受乙醇诱导的胃损伤，并激活前列腺素和CGRP机制。
