Michaloudi H C, Papadopoulos G C
Department of Anatomy, Veterinary School, University of Thessaloniki, Greece.
J Anat. 1996 Oct;189 ( Pt 2)(Pt 2):273-83.
Immunocytochemistry with antisera against serotonin (5-HT), dopamine (DA) and noradrenaline (NA) was used to detect monoaminergic (MA) fibres in the ventricular system of the hedgehog Erinaceus europaeus. Light microscopic examination of immunocytochemically stained sections revealed that the ventricular system of the hedgehog is unique among mammals in that the choroid plexuses receive CA axons and that the supraependyma and subependyma of the cerebral ventricles and the spinal central canal are innervated both by serotoninergic and catecholaminergic (CA) fibres. Supraependymal 5-HT axons were generally more abundant and created at places a large number of interconnected basket-like structures, whereas CA fibres were usually directed towards the ventricular lumen. In the lateral ventricles, CA fibres were more numerous in the ependyma lining grey matter, whereas a higher 5-HT innervation density was observed in the area between the corpus callosum and the caudate nucleus or the septum. In the 3rd ventricle, the ependyma of its dorsal part exhibited a higher 5-HT and NA innervation density, whereas DA fibres were preferentially distributed in the ventral half of the basal region. The ependyma lining the cerebral aqueduct displayed a higher MA innervation density in its ventral part. The ependymal wall of the 4th ventricle exhibited an extremely dense 5-HT innervation, mainly in the floor of the ventricle, relatively fewer NA fibres and only sparse DA ones. Few NA and relatively more 5-HT fibres were detected in the ependyma of the central canal. Finally, the circumventricular organs were unevenly innervated by the 3 types of MA fibres. The extensive monoaminergic innervation of the hedgehog ventricular system described here probably reflects a transitory evolutionary stage in the phylogeny of the MA systems with presently unknown functional implications.
利用抗血清对血清素（5-羟色胺，5-HT）、多巴胺（DA）和去甲肾上腺素（NA）进行免疫细胞化学检测，以在刺猬（欧洲刺猬）的脑室系统中检测单胺能（MA）纤维。对免疫细胞化学染色切片进行光学显微镜检查发现，刺猬的脑室系统在哺乳动物中独具特色，脉络丛接受儿茶酚胺能（CA）轴突，并且脑室的室管膜上层和室管膜下层以及脊髓中央管均由血清素能和儿茶酚胺能（CA）纤维支配。室管膜上层的5-HT轴突通常更为丰富，在某些部位形成大量相互连接的篮状结构，而CA纤维通常朝向脑室腔。在侧脑室中，灰质衬里的室管膜中CA纤维较多，而在胼胝体与尾状核或隔膜之间的区域观察到更高的5-HT神经支配密度。在第三脑室中，其背侧部分的室管膜表现出更高的5-HT和NA神经支配密度，而DA纤维优先分布在基部区域的腹侧半部。中脑导水管衬里的室管膜在其腹侧部分表现出更高的MA神经支配密度。第四脑室的室管膜壁表现出极其密集的5-HT神经支配，主要在脑室底部，NA纤维相对较少，DA纤维仅稀疏分布。在中央管的室管膜中检测到少量NA纤维和相对较多的5-HT纤维。最后，室周器官由这3种MA纤维的神经支配不均衡。此处描述的刺猬脑室系统广泛的单胺能神经支配可能反映了MA系统系统发育中的一个过渡进化阶段，其功能意义目前尚不清楚。
