Kay B H, Hearnden M N, Oliveira N M, Sellner I N, Hall R A
Queensland Institute of Medical Research, Royal Brisbane Hospital, Australia.
J Am Mosq Control Assoc. 1996 Sep;12(3 Pt 1):421-8.
This study addresses the potential problem of alphavirus infection associated with recreational use of the Ross River reservoir in north Queensland, Australia. From 1990 to 1993, 51,497 adult female mosquitoes were collected mainly by CO2-supplemented light traps. Four localities within the reservoir were considered and compared with mosquitoes collected during 1991 from 2 public localities around Townsville City. Ten isolates of Ross River virus, one of Barmah Forest virus, and 2 of Sindbis virus were recovered from Aedes normanensis, Anopheles amictus, and Culex annulirostris. All isolates were collected during the wet seasons of 1991 and 1992 using an enzyme immunoassay and cross-checked using a polymerase chain reaction assay Estimation of relative hazard was based on total mosquito abundance, prevalence of vector species, and on mosquito infection rates. Based on 1990-93 data, it was concluded that the Big Bay area of the Ross River dam, currently being considered as a primary recreational locality, was relatively safer than Antill Creek, Ross River, and Toonpan and presented no greater hazard than localities around urban Townsville, away from the reservoir. However, because of the changing ecology of the reservoir and lack of a full understanding of annual alphavirus activity, periodic surveillance is recommended.
本研究探讨了澳大利亚昆士兰州北部罗斯河水库休闲使用过程中与甲病毒感染相关的潜在问题。1990年至1993年期间，主要通过补充二氧化碳的诱蚊灯收集了51497只成年雌蚊。研究考虑了水库内的四个地点，并与1991年从汤斯维尔市周边两个公共地点收集的蚊子进行了比较。从诺曼伊蚊、骚扰按蚊和环纹库蚊中分离出10株罗斯河病毒、1株巴马森林病毒和2株辛德毕斯病毒。所有分离株均在1991年和1992年的雨季收集，采用酶免疫测定法，并使用聚合酶链反应测定法进行交叉核对。相对风险的评估基于蚊子的总数量、媒介种类的流行率以及蚊子的感染率。根据1990 - 1993年的数据得出结论，罗斯河大坝的大湾地区目前被视为主要的休闲地点，比安蒂尔溪、罗斯河和图潘相对更安全，且与远离水库的汤斯维尔市周边地区相比，风险并不更高。然而，由于水库生态环境的变化以及对甲病毒年度活动缺乏全面了解，建议进行定期监测。
