通过过表达非洲爪蟾NF-κB同源物XrelA对早期胚胎背侧模式形成进行负调控。

Negative regulation of dorsal patterning in early embryos by overexpression of XrelA, a Xenopus homologue of NF-kappa B.

作者信息

Kao K R, Lockwood A

机构信息

Terry Fox Cancer Research Laboratories, Division of Basic Medical Sciences, Memorial University of Newfoundland, St. John's, Canada.

出版信息

Mech Dev. 1996 Aug;58(1-2):129-39. doi: 10.1016/s0925-4773(96)00565-5.

DOI:10.1016/s0925-4773(96)00565-5

PMID:8887322

Abstract

Recent results by Richardson et al. (Mech. Dev., 52 (1995) 165-177) suggest that the Xenopus Rel gene XrelA may be involved in the formation of the head and tail of the early embryo. We present evidence to suggest that wild-type XrelA also has a role in dorsoventral development. XrelA overexpression in the dorsal side of embryos reduces dorsal development and attenuates in vitro dorsal morphogenetic movements. XrelA also strongly reduces axis duplication caused by overexpression of a dominant negative mutant of Xenopus glycogen synthase kinase-3 beta. Our results indicate that XrelA may have a role in dorsoventral patterning in early embryos.

摘要

理查森等人（《机制与发育》，第52卷（1995年），第165 - 177页）近期的研究结果表明，非洲爪蟾Rel基因XrelA可能参与早期胚胎头部和尾部的形成。我们提供的证据表明，野生型XrelA在背腹发育中也发挥作用。在胚胎背侧过表达XrelA会减少背侧发育，并减弱体外背侧形态发生运动。XrelA还能显著减少由非洲爪蟾糖原合酶激酶-3β的显性负性突变体过表达所导致的轴重复。我们的结果表明，XrelA可能在早期胚胎的背腹模式形成中发挥作用。