He X, Saint-Jeannet J P, Woodgett J R, Varmus H E, Dawid I B
Varmus Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Nature. 1995 Apr 13;374(6523):617-22. doi: 10.1038/374617a0.
Glycogen synthase kinase 3 (GSK-3) is homologous to the product of the Drosophila gene shaggy (zeste-white 3), which is required for signalling by wingless during Drosophila development. To test whether GSK-3 is also involved in vertebrate pattern formation, its role was investigated during early Xenopus development. It was found that dominant-negative GSK-3 mutants induced dorsal differentiation, whereas wild-type GSK-3 induced ventralization. These results indicate that GSK-3 is required for ventral differentiation, and suggest that dorsal differentiation may involve the suppression of GSK-3 activity by a wingless/wnt-related signal.
糖原合酶激酶3（GSK-3）与果蝇基因“蓬松”（小体-白色3）的产物同源，后者是果蝇发育过程中无翅信号传导所必需的。为了测试GSK-3是否也参与脊椎动物的模式形成，研究了其在非洲爪蟾早期发育过程中的作用。结果发现，显性负性GSK-3突变体诱导背侧分化，而野生型GSK-3诱导腹侧化。这些结果表明，GSK-3是腹侧分化所必需的，并提示背侧分化可能涉及无翅/翼状相关信号对GSK-3活性的抑制。
