Ando Y, Aoki A, Watanabe H, Ishikawa I
Department of Periodontology, Tokyo Medical and Dental University, Japan.
Lasers Surg Med. 1996;19(2):190-200. doi: 10.1002/(SICI)1096-9101(1996)19:2<190::AID-LSM11>3.0.CO;2-B.
Recently Erbium (Er) YAG laser has been developed for dentistry. It may be suitable for periodontal therapy. This study examined the bactericidal effect of the Er: YAG laser on periodontopathic bacteria in vitro.
STUDY DESIGN/MATERIALS AND METHODS: After spreading the bacterial suspension of Porphyromonas gingivalis or Actinobacillus actinomycetemcomitans on agar plates, a single pulse laser was applied to the agar plates at the energy density of 0.04-2.6 J/cm2. The growth of the bacterial colonies on the lased agar plates was examined after anaerobic culture. P. gingivalis colonies were also individually exposed to the single pulse laser at the energy of 1.8-10.6 J/cm2. The colony forming units of the irradiated colonies were counted.
Growth inhibitory zones were found at the irradiated sites at the energy of about 0.3 J/cm2 and higher. The survival ratios of the viable bacteria in the lased P. gingivalis colonies decreased significantly at the energy of 7.1 and 10.6 J/cm2, as compared with that of the control.
These findings suggest that the Er:YAG laser has a high bactericidal potential at a low energy level.
最近铒（Er）钇铝石榴石激光已被开发用于牙科领域。它可能适用于牙周治疗。本研究在体外检测了铒钇铝石榴石激光对牙周病原菌的杀菌作用。
研究设计/材料与方法：将牙龈卟啉单胞菌或伴放线放线杆菌的细菌悬液涂布于琼脂平板后，以0.04 - 2.6 J/cm²的能量密度对琼脂平板施加单脉冲激光。厌氧培养后检测经激光照射的琼脂平板上细菌菌落的生长情况。牙龈卟啉单胞菌菌落也分别以1.8 - 10.6 J/cm²的能量暴露于单脉冲激光下。对照射后的菌落计数其菌落形成单位。
在能量约为0.3 J/cm²及更高时，在照射部位发现生长抑制区。与对照组相比，经激光照射的牙龈卟啉单胞菌菌落中活菌的存活率在能量为7.1和10.6 J/cm²时显著降低。
这些结果表明铒钇铝石榴石激光在低能量水平下具有较高的杀菌潜力。
