Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Daneshjoo Blvd, Daneshgah Square, Velenjak, Shahid Chamran Highway, Tehran, 19839-69411, Iran.
Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Daneshjoo Blvd, Daneshgah Square, Velenjak, Shahid Chamran Highway, Tehran, 19839-63113, Iran.
Lasers Med Sci. 2022 Oct;37(8):3259-3268. doi: 10.1007/s10103-022-03615-y. Epub 2022 Jul 30.
Decontamination of implant surfaces is important to the treatment of peri-implantitis. Er:YAG laser and air-powder abrasion system are regarded as the most effective means of decontamination of implant surfaces. The aim of this in vitro study was to compare the activity of human dental pulp stem cells (hDPSCs) cultured on decontaminated sandblasted titanium discs using Er:YAG laser irradiation and air-powder abrasion. Forty-five titanium discs were contaminated with Escherichia coli (E. coli) bacteria and fifteen titanium discs served as sterile control groups. Thirty contaminated titanium discs were decontaminated with Er:YAG laser or air-powder abrasion system and fifteen contaminated discs were used as contaminated control group. Afterwards, hDPSCs were seeded on all sixty experimental titanium discs. The effects of two decontamination tools on hDPSCs viability were evaluated by MTT assay. Alkaline phosphatase (ALP) activity assay, quantitative real-time PCR analysis and alizarin red staining method were performed to assess hDPSCs osteogenic differentiation. Scanning microscope electron (SEM) was also used to evaluate the effects of two different decontaminated methods on cellular morphology. Our study showed that decontamination using Er:YAG laser caused maximum cell viability. However, the ALP activity was not different in laser and air-abrasion groups. The significant expression of an osteoblastic marker and stronger Alizarin red staining were observed in laser irradiation groups. In addition, SEM observation indicated that grown cells were more stretched and more filopodia in Er:YAG-treated discs. In the present study, Er:YAG laser and air-powder abrasion improved the activity of the cells cultured on the decontaminated titanium discs. However, in comparison with air-powder abrasion, Er:YAG laser was more effective.
器械表面的去污处理对于治疗种植体周围炎非常重要。Er:YAG 激光和喷沙系统被认为是器械表面去污的最有效手段。本体外研究的目的是比较经 Er:YAG 激光照射和喷沙处理后，人牙髓干细胞（hDPSCs）在去污喷砂钛片表面的活性。将 45 个钛片用大肠杆菌（E. coli）污染，15 个钛片作为无菌对照。用 Er:YAG 激光或喷沙系统对 30 个污染钛片进行去污处理，15 个污染钛片作为污染对照组。然后，将 hDPSCs 接种于所有 60 个实验用钛片上。通过 MTT 检测评价两种去污工具对 hDPSCs 活力的影响。通过碱性磷酸酶（ALP）活性检测、实时定量 PCR 分析和茜素红染色法评估 hDPSCs 成骨分化情况。扫描电镜（SEM）也用于评价两种不同去污方法对细胞形态的影响。本研究表明，Er:YAG 激光去污处理后的细胞活力最高。然而，激光和喷沙组的 ALP 活性无差异。激光照射组观察到成骨细胞标志物的显著表达和更强的茜素红染色。此外，SEM 观察表明，在 Er:YAG 处理的钛片上培养的细胞生长得更加伸展，有更多的丝状伪足。在本研究中，Er:YAG 激光和喷沙系统提高了在去污钛片上培养的细胞的活性。然而，与喷沙系统相比，Er:YAG 激光的效果更显著。
