缺氧/复氧后星形胶质细胞和内皮细胞之间过氧化氢释放的差异反应。

Differential response in the release of hydrogen peroxide between astroglial cells and endothelial cells following hypoxia/reoxygenation.

作者信息

Kondo T, Kinouchi H, Kawase M, Yoshimoto T

机构信息

Department of Neurosurgery, Tohoku University School of Medicine, Sendai, Japan.

出版信息

Neurosci Lett. 1996 Sep 6;215(2):103-6.

PMID:8888006

Abstract

Hydrogen peroxide (H2O2) and lactate dehydrogenase (LDH) released from cultured astroglial cells (AGs) and brain endothelial cells (BECs) were measured following hypoxia/reoxygenation. AGs released H2O2 to the extracellular space from 8 h of hypoxia with the cellular injury indicated by LDH increase. In contrast, BECs released H2O2 at 4 h of hypoxia, prior to the LDH increase. These results suggest that AGs and BECs play different roles in production and metabolism of oxygen free radicals in the blood-brain barrier (BBB), and AGs may protect the BBB disruption from oxidative stress after hypoxia/reoxygenation.

摘要

在缺氧/复氧后，对培养的星形胶质细胞（AGs）和脑内皮细胞（BECs）释放的过氧化氢（H2O2）和乳酸脱氢酶（LDH）进行了测量。从缺氧8小时起，AGs就向细胞外空间释放H2O2，同时LDH增加表明细胞受到损伤。相比之下，BECs在缺氧4小时就释放H2O2，早于LDH增加。这些结果表明，AGs和BECs在血脑屏障（BBB）中氧自由基的产生和代谢中发挥不同作用，并且AGs可能保护BBB在缺氧/复氧后免受氧化应激破坏。